Mercado-Pichardo E, Vilar-Rojas C, Wens-Flores M D, Bernal-Torres A
Arch Invest Med (Mex). 1981;12(1):107-14.
Experimental conditions were determined to find out if washed spermatozoids from fertile, health donors are capable of nitroblue tetrazolium (NBT) reduction in a similar way (although in the absence of a substrate) to that of leukocytes. Formazan blue formation obtained suggest the participation of the NADH oxidoreductase mitochondrial enzyme. The sperm capacity for NBT reduction might be take as a reflection of human sperm metabolic capacity. Our results seem to be useful for comparison of reduction (metabolic) capacity of normal sperms with that of individuals with fertility problems.
确定实验条件是为了弄清楚来自生育能力正常的健康供体的洗涤精子是否能够以与白细胞类似的方式（尽管没有底物）进行硝基蓝四氮唑（NBT）还原。所获得的甲臜蓝形成表明NADH氧化还原酶线粒体酶的参与。精子的NBT还原能力可能被视为人类精子代谢能力的一种反映。我们的结果似乎有助于比较正常精子与有生育问题个体的还原（代谢）能力。
