Miura Y, Urabe H, Miyamoto K, Okazaki M
Artif Organs. 1981 Feb;5(1):72-9.
Detoxication of ammonia was performed by dialysis of ammonia through a hollow fiber unit and by conversion to urea in a column reactor packed with immobilized urea cycle enzymes. Enzymes in the urea cycle could be immobilized by covalently binding on CNBr-activated Sepharose 4B. Ammonia was sequentially converted to urea by immobilized urea cycle enzymes with an ATP-regenerating system. Optimum conditions for the cyclic reaction were obtained theoretically and experimentally for performing continuous urea synthesis in a packed column reactor. Detoxication of ammonia of similar concentration to that in the blood in hepatic insufficiency was analyzed and performed experimentally in an extracorporeal model.
氨的解毒通过氨透过中空纤维装置的透析以及在填充固定化尿素循环酶的柱式反应器中转化为尿素来实现。尿素循环中的酶可以通过共价结合在溴化氰活化的琼脂糖4B上进行固定化。氨通过固定化尿素循环酶与ATP再生系统依次转化为尿素。从理论和实验上获得了在填充柱式反应器中进行连续尿素合成的循环反应的最佳条件。在体外模型中分析并实验进行了与肝功能不全患者血液中浓度相似的氨的解毒。
