Miura Y, Miyamoto K, Raze J M, Urabe H
J Biomed Mater Res. 1977 Sep;11(5):743-54. doi: 10.1002/jbm.820110509.
Carbamoylphosphate synthetase (EC 2.7.2.5) and ornithine carbamoyl-transferase (EC 2.1.3.3) extracted from frog liver were successfully immobilized on CNBr-activated Sepharose 4B. The immobilized preparation had a better stability towards heat. The apparent Michaelis constant values for N-acetylglutamate, ammonia, and ATP were not significantly changed by immobilization.
从蛙肝中提取的氨甲酰磷酸合成酶（EC 2.7.2.5）和鸟氨酸氨甲酰转移酶（EC 2.1.3.3）成功固定在溴化氰活化的琼脂糖4B上。固定化制剂对热具有更好的稳定性。固定化后，N-乙酰谷氨酸、氨和ATP的表观米氏常数没有显著变化。
