Nicoll A, Duffield R, Lewis B
Atherosclerosis. 1981 May;39(2):229-42. doi: 10.1016/0021-9150(81)90073-3.
Arterial specimens were obtained from 12 patients during arterial repair surgery and 3 patients during nephrectomy following a bolus injection of autologous radio-iodinated very low density lipoprotein (VLDL) up to 30 h before surgery. Radioactivity in apoprotein B (apo B) was determined in lipoprotein fractions isolated from saline extracts of washed intima in the density ranges less than 1.006 g/ml, 1.006-1.019 g/ml and 1.019-1.063 g/ml [intimal VLDL, intermediate-(IDL) and low density lipoprotein (LDL), respectively]. Specific radioactivity of apo B was measured in corresponding plasma protein fractions. In atheromatous intima, minimum influx rates of VLDL-apo B [12.1 +/- 4.3 (SEM) ng . cm-1 . h-1] and IDL-apo B (13.6 +/- 5.1) over 24 h were significantly higher than fluxes of VLDL-apo B (1.2 +/- 0.34) and IDL-apo B (1.4 +/- 1.1) into normal intima (P less than 0.05). Minimum influx rate of LDL-apo B was also significantly higher in atheromatous intima (196 +/- 39) compared with normal intima (25.8 +/- 13.5, P less than 0.01) and was 16-18 fold higher than corresponding VLDL- and IDL-apo B fluxes in both types of intima. Collagenase digestion of intimal samples after saline extraction yielded further significant mass of VLDL but this fraction had little radioactivity compared with saline extractable VLDL.
在12例患者进行动脉修复手术期间以及3例患者进行肾切除手术期间获取动脉标本，手术前高达30小时给予自体放射性碘化极低密度脂蛋白（VLDL）大剂量注射。在手术前，从密度范围小于1.006 g/ml、1.006 - 1.019 g/ml和1.019 - 1.063 g/ml的冲洗内膜盐水提取物中分离的脂蛋白组分中测定载脂蛋白B（apo B）的放射性[分别为内膜VLDL、中间密度脂蛋白（IDL）和低密度脂蛋白（LDL）]。在相应的血浆蛋白组分中测量apo B的比放射性。在动脉粥样硬化内膜中，VLDL-apo B[12.1±4.3（SEM）ng·cm⁻¹·h⁻¹]和IDL-apo B（13.6±5.1）在24小时内的最小流入速率显著高于VLDL-apo B（1.2±0.34）和IDL-apo B（1.4±1.1）进入正常内膜的通量（P<0.05）。与正常内膜（25.8±13.5，P<0.01）相比，动脉粥样硬化内膜中LDL-apo B的最小流入速率也显著更高，并且比两种类型内膜中相应的VLDL-和IDL-apo B通量高16 - 18倍。盐水提取后内膜样品的胶原酶消化产生了更多的VLDL，但与盐水可提取的VLDL相比，该组分的放射性较低。
