Celik C, Mittelman A, Paolini N S, Lewis D, Evans J T
Cancer Res. 1981 Jul;41(7):2908-11.
In this experiment, a segment of the left colon including the upper part of the rectum was transposed to the upper jejunum, and a segment of upper jejunum was transposed to the left colon of the same animal. In another group, the same colon and jejunum segments were transsected and reanastomosed in place. A third group served as a normal control. After a recovery period, weekly s.c. 1,2-symmetrical dimethylhydrazine injections were begun. Each animal received a total of 20 injections at a dose of 20 mg/kg. Five weeks after the last 1,2-symmetrical dimethylhydrazine injection, 15 of 19 (79%) of the animals had one or more tumor(s) in the transposed colon segment, while none had tumor in the transposed jejunal segment. Transsected and reanastomosed animals showed the same distribution of tumors as did the normal control animals. All three groups had tumors at other sites in the colon and rectum. In addition, about 20% had tumors of the duodenojejunal area. These data indicate that the colonic mucosa is the primary target for the carcinogenic effect of 1,2-symmetrical dimethylhydrazine, independent of other variables such as the fecal stream.
在本实验中，将一段包括直肠上部的左结肠移植到空肠上段，同时将一段空肠上段移植到同一动物的左结肠。在另一组中，将相同的结肠段和空肠段切除后原位重新吻合。第三组作为正常对照组。经过恢复期后，开始每周皮下注射1,2 - 对称二甲基肼。每只动物共接受20次注射，剂量为20 mg/kg。在最后一次注射1,2 - 对称二甲基肼后5周，19只动物中有15只（79%）在移植的结肠段出现一个或多个肿瘤，而移植的空肠段未出现肿瘤。切除并重新吻合的动物肿瘤分布与正常对照动物相同。所有三组在结肠和直肠的其他部位均有肿瘤。此外，约20%的动物在十二指肠空肠区域有肿瘤。这些数据表明，结肠黏膜是1,2 - 对称二甲基肼致癌作用的主要靶点，与粪便流等其他变量无关。
