Mercer A R, McGregor D D
Cell Tissue Res. 1981;218(1):191-207. doi: 10.1007/BF00210104.
The innervation of the gut of the venerid bivalve mollusc, Chione stutchburyi, has been examined by fluorescence histochemistry, electron microscopy and autoradiography. Specific green and yellow varicose fluorescent fibres indicate the presence of dopaminergic and serotonergic axons, respectively. Three different types of axons can be distinguished by the morphological characteristics of their vesicles. Type I axons contain predominantly small granular vesicles (average diameter 65 nm), Type II axons possess large granular vesicles (average diameter 100 nm) and Type III axons contain large opaque vesicles (average diameter 150 nm). The granular vesicles in both Types I and II axons react positively to dichromate, and their granularity is reduced by reserpine indicating that they are monoaminergic. Only Type I axons accumulate tritiated dopamine and are selectively damaged by 6-hydroxydopamine. It is concluded that Type I axons are dopaminergic. Type II axons are serotonergic: they alone take up tritiated 5-hydroxytryptamine, and 5,7-dihydroxytryptamine selectively causes degenerative changes in these axons. Type III axons contain an unidentified neurotransmitter substance. The large opaque vesicles of these axons do not react to dichromate and are unaffected by reserpine, 6-hydroxydopamine or 5,7-dihydroxytryptamine.
通过荧光组织化学、电子显微镜和放射自显影技术，对帘蛤科双壳贝类软体动物斯氏缀锦蛤的肠道神经支配进行了研究。特定的绿色和黄色曲张荧光纤维分别表明存在多巴胺能和5-羟色胺能轴突。根据其囊泡的形态特征，可以区分出三种不同类型的轴突。I型轴突主要含有小颗粒囊泡（平均直径65纳米），II型轴突具有大颗粒囊泡（平均直径100纳米），III型轴突含有大的不透明囊泡（平均直径150纳米）。I型和II型轴突中的颗粒囊泡对重铬酸盐呈阳性反应，并且它们的颗粒度会因利血平而降低，这表明它们是单胺能的。只有I型轴突积累氚化多巴胺，并被6-羟基多巴胺选择性损伤。得出结论，I型轴突是多巴胺能的。II型轴突是5-羟色胺能的：只有它们摄取氚化5-羟色胺，并且5,7-二羟基色胺会选择性地导致这些轴突发生退行性变化。III型轴突含有一种未鉴定的神经递质物质。这些轴突的大不透明囊泡对重铬酸盐无反应，并且不受利血平、6-羟基多巴胺或5,7-二羟基色胺的影响。
