Wullems G J, Molendijk L, Ooms G, Schilperoort R A
Cell. 1981 Jun;24(3):719-27. doi: 10.1016/0092-8674(81)90098-2.
Tumorous tobacco shoots have been derived from callus tissues produced by Agrobacterium tumefaciens--induced transformation of tobacco protoplasts and by fusion of normal protoplasts with those from crown gall tumors. The continued presence of T-DNA sequences in shoots is directly demonstrated by Southern blotting and is also revealed by the presence of the tumor markers octopine and nopaline. When grafted onto normal tobacco plants, both octopine- and nopaline-type shoots (including those from somatic hybrids) produced flowers and set seed. Germination of these seeds gave F1 progeny that showed retention of morphological markers of their parental shoots, and one seedling retained the ability to synthesize nopaline. The data demonstrate that T-DNA markers can be retained during meiosis and are expressed in F1 plants.
肿瘤性烟草芽是由根癌农杆菌诱导烟草原生质体转化以及正常原生质体与冠瘿瘤原生质体融合产生的愈伤组织发育而来。通过Southern印迹法直接证明了芽中T-DNA序列的持续存在，并且肿瘤标记物章鱼碱和胭脂碱的存在也揭示了这一点。当嫁接到正常烟草植株上时，章鱼碱型和胭脂碱型芽（包括体细胞杂种的芽）都能开花并结籽。这些种子的萌发产生了F1后代，其表现出亲本芽形态标记的保留，并且有一株幼苗保留了合成胭脂碱的能力。数据表明，T-DNA标记在减数分裂过程中可以保留并在F1植株中表达。
