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植物和烟草组织中的与发病机制相关的蛋白质,经根癌农杆菌转化。

Pathogenesis-related proteins in plants and tissues ofNicotiana tabacum transformed byAgrobacterium tumefaciens.

机构信息

Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts, UK.

出版信息

Plant Mol Biol. 1983 Nov;2(6):317-20. doi: 10.1007/BF01578593.

DOI:10.1007/BF01578593
PMID:24318451
Abstract

Large amounts of pathogenesis-related (PR) proteins were found inNicotiana tabacum crown gall tissue, following transformation of normal tobacco cells withAgrobacterium tumefaciens. In contrast, PR proteins were not detected in leaves of grafted plants that had been recovered from crown gall tissue even though these plants were still transformed as shown by their inability to form roots and ability to produce octopine. No difference was observed in susceptibility to virus infection between untransformed and transformed plants grafted onto identical rootstocks. The results are discussed in relation to physiological factors controlling PR protein induction and virus resistance.

摘要

在将正常烟草细胞用根癌农杆菌转化后,烟草冠瘿组织中发现了大量与发病机理相关(PR)的蛋白质。相比之下,即使在已从冠瘿组织中恢复的嫁接植株的叶片中也未检测到 PR 蛋白,但这些植株仍然被转化,因为它们无法形成根且能够产生章鱼碱。在接种到相同砧木上的未转化和转化植株之间,对病毒感染的敏感性没有差异。该结果与控制 PR 蛋白诱导和病毒抗性的生理因素有关。

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Pathogenesis-related proteins in plants and tissues ofNicotiana tabacum transformed byAgrobacterium tumefaciens.植物和烟草组织中的与发病机制相关的蛋白质,经根癌农杆菌转化。
Plant Mol Biol. 1983 Nov;2(6):317-20. doi: 10.1007/BF01578593.
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T-DNA organization in homogeneous and heterogeneous octopine-type crown gall tissues of Nicotiana tabacum.烟草八氢番茄红素型冠瘿组织中T-DNA在同质性和异质性组织中的结构
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本文引用的文献

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Acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco.乙酰水杨酸(阿司匹林)可诱导烟草对烟草花叶病毒产生抗性。
Virology. 1979 Dec;99(2):410-2. doi: 10.1016/0042-6822(79)90019-9.
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Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. 'Samsun' and 'Samsun NN'. IV. Similarity of qualitative changes of specific proteins after infection with different viruses and their relationship to acquired resistance.烟草品种“萨姆松”和“萨姆松NN”叶片可溶性蛋白的聚丙烯酰胺圆盘电泳。IV. 不同病毒感染后特定蛋白质定性变化的相似性及其与获得性抗性的关系。
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Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA.
冠瘿瘤中的细胞分裂素/生长素平衡受 T-DNA 中特定基因座的调节。
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DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
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DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
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Retention of tumor markers in F1 progeny plants from in vitro induced octopine and nopaline tumor tissues.体外诱导的章鱼碱型和胭脂碱型肿瘤组织的F1代子代植株中肿瘤标志物的保留情况。
Cell. 1981 Jun;24(3):719-27. doi: 10.1016/0092-8674(81)90098-2.
7
[Hypersensitivity to viruses, temperature and soluble proteins in Nicotiana Xanthi n.c. Appearance of new macromolecules at the repression of viral synthesis].[烟草品种Xanthi n.c.对病毒、温度和可溶性蛋白质的过敏反应。病毒合成受抑制时新大分子的出现]
C R Acad Hebd Seances Acad Sci D. 1970 May 11;270(19):2383-6.
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Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. "Samsun" and "Samsun NN". II. Changes in protein constitution after infection with tobacco mosaic virus.烟草品种“萨姆逊”和“萨姆逊NN”叶片可溶性蛋白的聚丙烯酰胺圆盘电泳。II. 感染烟草花叶病毒后蛋白质组成的变化
Virology. 1970 Feb;40(2):190-211. doi: 10.1016/0042-6822(70)90395-8.
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Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.质粒DNA稳定整合到高等植物细胞中:冠瘿瘤形成的分子基础。
Cell. 1977 Jun;11(2):263-71. doi: 10.1016/0092-8674(77)90043-5.
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Induction by 2-chloroethylophosphonic acid of viral-like lesions, associated proteins, and systemic resistance in tobacco.2-氯乙基膦酸诱导烟草产生类病毒病斑、相关蛋白及系统抗性
Virology. 1977 Jul 15;80(2):417-20. doi: 10.1016/s0042-6822(77)80016-0.