Milo G E, Weisbrode S A, Zimmerman R, McCloskey J A
Chem Biol Interact. 1981 Jul;36(1):45-59. doi: 10.1016/0009-2797(81)90028-4.
Human foreskin cell cultures in scheduled DNA synthesis (S phase) of the cell cycle were exposed to UV irradiation at a dose of 10 J . m-1 in the presence of insulin. These treated cell populations, when selectively passaged in a high amino acid supplemented complete growth medium (CM) after 20 Dulbecco's phosphate buffered saline (pH 6.8) (PDL), were able to be grown in soft agar. These treated cell populations were also grown in 1% serum supplemented growth medium and at 41 degrees C in 10% serum supplemented growth medium. Cell populations 4--5 PDL after treatment exhibited altered colony morphology and altered lectin agglutination profiles but would not grow in soft agar. These events appeared to be associated with the early stages in the expression phase of the transformed phenotype. After 20 PDL, we observed that these cells would grow in soft agar at a frequency of 20 colonies/10(5) cells seeded in soft agar. The cell populations derived from these colonies, when propagated and injected into the nude mice, formed myxofibromas at the injection sites rather than the type of tumor (fibrosarcoma) previously described for chemical carcinogen-induced neoplasms.
处于细胞周期中预定DNA合成（S期）的人包皮细胞培养物在胰岛素存在的情况下接受剂量为10 J·m⁻¹的紫外线照射。这些经过处理的细胞群体在20次杜尔贝科磷酸盐缓冲盐水（pH 6.8）（PDL）处理后，在补充了高氨基酸的完全生长培养基（CM）中进行选择性传代培养时，能够在软琼脂中生长。这些经过处理的细胞群体也在补充了1%血清的生长培养基中以及在41℃下补充了10%血清的生长培养基中培养。处理后4 - 5 PDL的细胞群体表现出改变的集落形态和改变的凝集素凝集谱，但不能在软琼脂中生长。这些事件似乎与转化表型表达阶段的早期有关。20次PDL后，我们观察到这些细胞能够在软琼脂中生长，接种到软琼脂中的细胞形成集落的频率为每10⁵个细胞产生20个集落。从这些集落衍生的细胞群体在繁殖并注射到裸鼠体内后，在注射部位形成黏液纤维瘤，而不是先前描述的化学致癌物诱导肿瘤的肿瘤类型（纤维肉瘤）。
