Su Z Z, Luo Z Y, Guo L P, Lu S Z, Liu Y L
Department of Biology, Fudan University, Shanghai.
Sci Sin B. 1988 Jul;31(7):850-9.
Treatment of early passage human fetal gastric fibroblasts with ultraviolet (UV) light and the chemical carcinogen ethyl nitrosourea (ENU) in succession resulted in an immortally growing cell line, named GTS 8502. The cells of this line display typical transformation characteristics, such as irregularly shaped nuclei, heteroploidization of karyotype and frequent appearance of heteromorphic chromosomes, the enhanced volume ratio of nucleus to cytoplasm, multinucleoli, appearance of microvilli on the surface of the cells and agglutination reaction to lectin concanavalin A. The transformants have high growing and mitotic indices and the ability of focus-formation on monolayers and anchorage independent growth in soft agar medium. Moreover, these cells induced tumours in nude mice or in immunosuppressed new-born rats through heterotransplantation. The results of various methods including electromicroscopy and histochemical analyses indicate that GTS 8502 cells are of fibroblast origin. Our results thus indicate that synergism of two carcinogens may raise the transformation ratio of normal human cells, which is otherwise extremely low. The transformation of human cells may be utilized to detect environmental carcinogens.
用紫外线（UV）和化学致癌物亚硝基脲（ENU）先后处理早期传代的人胎儿胃成纤维细胞，得到了一个永生化生长的细胞系，命名为GTS 8502。该细胞系的细胞表现出典型的转化特征，如细胞核形状不规则、核型异倍体化以及异形染色体频繁出现、核质体积比增加、多核仁、细胞表面出现微绒毛以及对凝集素刀豆球蛋白A发生凝集反应。转化细胞具有高生长指数和有丝分裂指数，能够在单层上形成集落，并在软琼脂培养基中进行非贴壁依赖性生长。此外，这些细胞通过异种移植在裸鼠或免疫抑制新生大鼠中诱发肿瘤。包括电子显微镜和组织化学分析在内的各种方法的结果表明，GTS 8502细胞起源于成纤维细胞。因此，我们的结果表明，两种致癌物的协同作用可能会提高正常人细胞的转化率，否则该转化率极低。人细胞的转化可用于检测环境致癌物。
