Halls D J, Fell G S, Dunbar P M
Clin Chim Acta. 1981 Jul 18;114(1):21-7. doi: 10.1016/0009-8981(81)90223-0.
Two methods are described for the determination of copper in urine by graphite furnace atomic absorption spectrometry. The first, suitable for spectrometers capable of making good background correction at 325 nm, involves a direct determination against simple aqueous standards after 2 X dilution of the sample. The second requires no background correction and can be used when background correction is not available or is inadequate. The copper is extracted from the urine with ammonium tetramethylenedithiocarbamate into methylisobutylketone and the extract is analysed for copper. No significant matrix effect was observed for either method. Analysis of random urine samples from 16 normal subjects gave a mean concentration of 0.42 mumol/l with a range of 0.06--1.04 mumol/l.
本文描述了两种用石墨炉原子吸收光谱法测定尿液中铜含量的方法。第一种方法适用于能够在325nm处进行良好背景校正的光谱仪，该方法是在将样品稀释2倍后，直接与简单的水溶液标准品进行对比测定。第二种方法不需要背景校正，当无法进行背景校正或背景校正不充分时可以使用。用四亚甲基二硫代氨基甲酸铵从尿液中萃取铜，萃取到甲基异丁基甲酮中，然后对萃取液进行铜含量分析。两种方法均未观察到明显的基体效应。对16名正常受试者的随机尿液样本进行分析，得到的平均浓度为0.42μmol/L，范围为0.06 - 1.04μmol/L。
