Anderson R H, Ho S Y, Smith A, Wilkinson J L, Becker A E
Diagn Histopathol. 1981 Jan-Mar;4(1):3-15.
We have reviewed our experience with study of the conduction system of the heart and tried to condense our experience in the hope of aiding others to study this neglected part of the heart. The techniques involved are not complicated but do require patience and attention to detail. The key to success is the ability to cut serial sections, and to mount and study sub-serial sections. The techniques used are briefly described and then attention is given to the removal of blocks, the single most important step in the whole procedure. Advice is offered on the optimal method of studying the sinus node and the atrioventricular junctional area in the normal heart. Thereafter methods are described for studying the conduction systems in hearts with abnormal atrioventricular nodes, with ventricular septal defects and with ventricular pre-excitation. Similar attention is then paid to sudden infant death syndrome with regard to the conduction system, to congenitally complete heart block and finally to acquired disease in congenitally malformed hearts. Hope is expressed that more may become interested in the study of this fascinating system, or else refer suitable hearts to those who already are.
我们回顾了我们对心脏传导系统的研究经验,并试图总结我们的经验,以期帮助其他人研究心脏这一被忽视的部分。所涉及的技术并不复杂,但确实需要耐心和注重细节。成功的关键在于能够切出连续切片,并对亚连续切片进行裱贴和研究。文中简要描述了所使用的技术,然后重点介绍了取材这一整个过程中最重要的一步。针对正常心脏中窦房结和房室交界区的最佳研究方法提供了建议。此后,还描述了研究房室结异常、室间隔缺损和心室预激心脏传导系统的方法。接着,对婴儿猝死综合征的传导系统、先天性完全性心脏传导阻滞以及先天性畸形心脏的后天性疾病给予了类似的关注。我们希望有更多人能对这个迷人的系统研究产生兴趣,或者将合适的心脏标本提供给已经感兴趣的人。