Hawkins E F, Lieskovsky G, Markland F S
J Clin Endocrinol Metab. 1981 Aug;53(2):456-8. doi: 10.1210/jcem-53-2-456.
Using the sucrose density gradient technique, we have compared androgen receptor values obtained in the presence and absence of sodium molybdate from cytosols of surgically obtained human prostate cancer tissue. In all nine prostates examined, inclusion of molybdate in the buffers resulted in an increase in the amount of androgen receptor detected, in some cases dramatically. Since accurate androgen receptor assays could be valuable for therapeutic decision making in prostate cancer, we advocate the inclusion of sodium molybdate in assay buffers to guard against the possibility of false negative or artificially low values.
利用蔗糖密度梯度技术，我们比较了在有和没有钼酸钠存在的情况下，从手术获取的人类前列腺癌组织胞液中获得的雄激素受体值。在所检测的全部9个前列腺样本中，缓冲液中加入钼酸钠会导致检测到的雄激素受体量增加，在某些情况下增加显著。由于准确的雄激素受体检测对于前列腺癌的治疗决策可能很有价值，我们主张在检测缓冲液中加入钼酸钠，以防出现假阴性或人为低值的可能性。
