用于分析胞浆抗原的免疫电泳技术比较

Comparison of immunoelectrophoretic techniques for the analysis of cytosol antigens.

作者信息

Chakrabarty S, Taylor C W, Yeoman L C

出版信息

J Immunol Methods. 1981;43(3):301-11. doi: 10.1016/0022-1759(81)90178-2.

Abstract

Crossed immunoelectrophoresis was employed in the analysis of cytosol antigens of a human colon adenocarcinoma cell line. Other immunoelectrophoretic techniques - crossed immunoelectrophoresis in the presence of Ampholine (incorporation of Ampholine in the first dimension electrophoresis gel), crossed immunotachophoresis and crossed immunoelectrofocusing - were also investigated ad compared with crossed immunoelectrphoresis in an attempt to select the optimal immunoelectrophoretic system for the analysis of cytosol antigens. The results of these comparisons showed that each technique offered its own advantages. The maximum number of immunoprecipitin peaks were detected by crossed immunoelectrophoresis. Both crossed immunoelectrophoresis in the presence of Ampholine and crossed immunotachophoresis provided the greatest resolution of electrophoretically similar antigens. Crossed immunoelectrofocusing provided IP values for these antigens. It was concluded that these techniques, when employed in combination, provided a more complete analysis of the complex cytosol antigenic mixture and may be useful when employed in combination to other antigen-antibody systems.

摘要

采用交叉免疫电泳分析人结肠腺癌细胞系的胞质溶胶抗原。还研究了其他免疫电泳技术——在两性电解质存在下的交叉免疫电泳（在第一维电泳凝胶中加入两性电解质）、交叉免疫层析电泳和交叉免疫电聚焦，并与交叉免疫电泳进行比较，以试图选择用于分析胞质溶胶抗原的最佳免疫电泳系统。这些比较结果表明，每种技术都有其自身的优势。通过交叉免疫电泳检测到的免疫沉淀峰数量最多。在两性电解质存在下的交叉免疫电泳和交叉免疫层析电泳都能对电泳相似的抗原提供最大分辨率。交叉免疫电聚焦为这些抗原提供了IP值。得出的结论是，这些技术联合使用时，能对复杂的胞质溶胶抗原混合物进行更全面的分析，并且与其他抗原-抗体系统联合使用时可能会很有用。