Hornsleth A, Mordhorst C H, Pedersen I R
J Immunol Methods. 1980;39(1-2):95-100. doi: 10.1016/0022-1759(80)90298-7.
The crossed immunoelectrophoretic technique was employed for the production of subtype-specific influenza A virus antisera. Glycoprotein-containing precipitates were produced by this technique by using detergent solubilized influenza A virus glycoproteins as antigens in the first dimension gel and rabbit antisera against these antigens in the second dimension gel. By cutting these precipitates out of the gel and using them for the immunization of rabbits, antisera were produced which showed the presence of subtype-specific antibodies in haemagglutination inhibition and immunofluorescent antibody tests but no antibodies in neuraminidase inhibition tests.
采用交叉免疫电泳技术制备甲型流感病毒亚型特异性抗血清。在该技术中，通过使用去污剂溶解的甲型流感病毒糖蛋白作为第一维凝胶中的抗原以及兔抗这些抗原的血清作为第二维凝胶中的抗体，产生了含糖蛋白的沉淀。通过从凝胶中切下这些沉淀并将其用于免疫兔子，制备出的抗血清在血凝抑制试验和免疫荧光抗体试验中显示存在亚型特异性抗体，但在神经氨酸酶抑制试验中未显示有抗体。
