绵羊和牛的金属硫蛋白：纯化、种类数量、锌含量及氨基酸组成

Ovine and bovine metallothioneins: purification, number of species, zinc content and amino acid composition.

作者信息

Whanger P D, Oh S H, Deagen J T

出版信息

J Nutr. 1981 Jul;111(7):1207-15. doi: 10.1093/jn/111.7.1207.

DOI:10.1093/jn/111.7.1207

Abstract

Metallothioneins (MTs) were purified with respect to zinc from tissues of cattle and sheep which had been fed high levels of dietary zinc. These proteins were purified by gel filtration (Sephadex G-75), ion exchange chromatography (twice on DEAE Sephacel) and gel filtration again (Bio-Gel P-10). There is one major and up to three minor MT species in liver, kidney and pancreas from both sheep and cattle. The amount of zinc varies between the different species of MT. Amino acid analyses of the purified preparations revealed a cysteine content of up to 35.7% and the absence of aromatic amino acids for the major species, but the minor proteins contained less cysteine and some aromatic amino acids. The significance of MT in zinc metabolism is discussed.

摘要

从摄入高剂量膳食锌的牛和羊的组织中，针对锌对金属硫蛋白（MTs）进行了纯化。这些蛋白质通过凝胶过滤（葡聚糖凝胶G - 75）、离子交换色谱法（在DEAE - 葡聚糖凝胶上进行两次）以及再次凝胶过滤（生物凝胶P - 10）进行纯化。绵羊和牛的肝脏、肾脏和胰腺中存在一种主要的MT种类以及多达三种次要的MT种类。不同种类的MT中锌含量各不相同。对纯化制剂的氨基酸分析显示，主要种类的半胱氨酸含量高达35.7%且不含芳香族氨基酸，但次要蛋白质所含半胱氨酸较少且含有一些芳香族氨基酸。文中讨论了MT在锌代谢中的意义。