Floriańczyk Bolesław
Department of Biochemistry and Molecular Biology, Medical University of Lublin.
Ann Univ Mariae Curie Sklodowska Med. 2003;58(2):91-4.
Metallothioneins (MTs) are known as heat-stable proteins and are able to be precipitated at 100 degrees C for 2 min. The aim of this work was to determine the content of metallothioneins and zinc in fraction after gel filtration (Sephadex G-75). The research was supposed to answer the question whether metallothioneins contain zinc ions after heat-treated cytosol. The rabbit liver cytosol was initially heat-treated to remove the heat-liable proteins and then cytosol was applied to a gel filtration column (Sephadex G-75). Metallothioneins content in each eluted fraction was measured by cadmium-hemoglobin affinity assay using the cadmium isotope (109Cd). Another experiment was conducted to further confirm whether the MTs peak contains zinc. A metal peak appeared at low molecular weight fractions. The metallothioneins isolated from rabbit liver contain zinc.
金属硫蛋白（MTs）是已知的热稳定蛋白，能够在100℃下沉淀2分钟。这项工作的目的是测定凝胶过滤（葡聚糖G - 75）后组分中金属硫蛋白和锌的含量。该研究旨在回答热处理后的细胞溶胶中的金属硫蛋白是否含有锌离子这一问题。兔肝细胞溶胶首先经过热处理以去除热不稳定蛋白，然后将细胞溶胶应用于凝胶过滤柱（葡聚糖G - 75）。使用镉同位素（109Cd）通过镉 - 血红蛋白亲和测定法测量每个洗脱组分中的金属硫蛋白含量。进行了另一项实验以进一步确认MTs峰是否含有锌。在低分子量组分中出现了一个金属峰。从兔肝中分离出的金属硫蛋白含有锌。
