Street K W, Schenk G H
J Pharm Sci. 1981 Jun;70(6):641-6. doi: 10.1002/jps.2600700617.
Spectrofluorometry, either direct or in combination with a separation technique, provides a sensitive and accurate method for the determination of certain extent fluorescent analgesic drugs and the determination of impurities in many combination preparations. A critical examination of the UV spectra of common analgesics and related compounds indicates that the fluorescence inner filter effect should be negligible below 10(-5) M and that selective excitation and emission wavelengths should minimize interference from other fluorescent species. Fluorometric procedures are presented for the determination of salicylamide, acetylsalicylic acid, and salicylic acid, as an impurity, in preparations containing salicylamide, acetylsalicylic acid, acetaminophen, caffeine, and phenacetin as major constituents. Inner filtering is the limiting factor only for the direct and indirect determination of salicylamide and the direct determination of acetylsalicylic acid. Results of fluorometric determinations compare favorably with other reference methods. Salicylic acid is determined in the 10(-7) M concentration range after separation from salicylamide, acetaminophen, and caffeine.
荧光分光光度法，无论是直接法还是与分离技术相结合，都为某些荧光镇痛药物的测定以及许多复方制剂中杂质的测定提供了一种灵敏且准确的方法。对常见镇痛药及相关化合物的紫外光谱进行的严格检查表明，在浓度低于10^(-5) M时，荧光内滤效应应可忽略不计，且选择性的激发和发射波长应能将来自其他荧光物质的干扰降至最低。本文介绍了荧光分析法，用于测定含有水杨酰胺、乙酰水杨酸、对乙酰氨基酚、咖啡因和非那西丁作为主要成分的制剂中，作为杂质的水杨酰胺、乙酰水杨酸和水杨酸。内滤作用仅是直接和间接测定水杨酰胺以及直接测定乙酰水杨酸时的限制因素。荧光测定结果与其他参考方法相比具有优势。从水杨酰胺、对乙酰氨基酚和咖啡因中分离后，可在10^(-7) M浓度范围内测定水杨酸。
