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谷氨酸脱氢酶信使核糖核酸的翻译及跟踪该酶生物合成的分光光度法。

Translation of mRNA for glutamate dehydrogenase and spectrophotometric procedure to follow the enzyme biosynthesis.

作者信息

Hillar M, Davis S A, Nikbakht K N, Chambers D

出版信息

Arch Int Physiol Biochim. 1977 Aug;85(3):583-8. doi: 10.3109/13813457709069874.

DOI:10.3109/13813457709069874
PMID:72534
Abstract

Heterogeneous poly (A)-mRNA fraction was isolated from rat liver microsomes using phenol-chloroform extraction, millipore filtration and poly (U)-agarose affinity chromatography. Obtained fractions were characterized with respect to their secondary structure and poly (A) content. Isolated poly (A)-mRNA fraction contained high template activity for glutamate dehydrogenase in cell-free systems with microsomes or polysomes. A spectrophotometric procedure to follow enzyme biosynthesis was also developed.

摘要

使用苯酚-氯仿萃取、微孔过滤和聚（U）-琼脂糖亲和色谱法从大鼠肝脏微粒体中分离出异质多聚（A）-mRNA 组分。对获得的组分的二级结构和多聚（A）含量进行了表征。分离出的多聚（A）-mRNA 组分在含有微粒体或多核糖体的无细胞体系中对谷氨酸脱氢酶具有高模板活性。还开发了一种用于跟踪酶生物合成的分光光度法。

相似文献

1
Translation of mRNA for glutamate dehydrogenase and spectrophotometric procedure to follow the enzyme biosynthesis.谷氨酸脱氢酶信使核糖核酸的翻译及跟踪该酶生物合成的分光光度法。
Arch Int Physiol Biochim. 1977 Aug;85(3):583-8. doi: 10.3109/13813457709069874.
2
Studies on a rat liver subcellular poly A-rich RNA fraction with glutamate dehydrogenase template activity.关于具有谷氨酸脱氢酶模板活性的大鼠肝脏亚细胞富含多聚腺苷酸RNA组分的研究。
Physiol Chem Phys. 1977;9(3):205-15.
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Control of transcription and translation by low molecular weight peptides (deprimerones) from chromatin and poly(A)-messenger RNA. Implication in the mechanism of carcinogenesis.
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Release of poly A(+) messenger RNA from rat liver rough microsomes upon disassembly of bound polysomes.结合多核糖体解体后大鼠肝脏粗面微粒体中多聚腺苷酸（+）信使核糖核酸的释放
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Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe.使用分光光度探针鉴定谷氨酸脱氢酶的信使核糖核酸
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Translation of glutamate dehydrogenase mRNA in a reticulocyte cell-free system.
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Different binding of poly(A)-containing and poly(A)-free fractions of nuclear ribonucleic acid to ribosomes from rat liver.大鼠肝脏核糖核酸中含聚腺苷酸和不含聚腺苷酸部分与核糖体的不同结合
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[Effect of hydrocortisone on the properties of rat liver polyribosomes, metabolism, and template activity of polysomal poly-A-containing RNA].[氢化可的松对大鼠肝脏多核糖体性质、代谢及多核糖体含多聚腺苷酸RNA模板活性的影响]
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Structure and function of rat liver polysome populations. II. Characterization of polyadenylate-containing mRNA associated with subpopulations of membrane-bound particles.大鼠肝脏多核糖体群体的结构与功能。II. 与膜结合颗粒亚群相关的含聚腺苷酸mRNA的特性
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