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使用分光光度探针鉴定谷氨酸脱氢酶的信使核糖核酸

Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe.

作者信息

Hillar M, Orudiakomo S

出版信息

Mol Biol Rep. 1986;11(3):131-5. doi: 10.1007/BF00419732.

DOI:10.1007/BF00419732
PMID:2876375
Abstract

Specific messenger RNA for glutamate dehydrogenase was partially purified from a calf liver polysomal poly(A)-mRNA fraction by sucrose density gradient centrifugation. Enzyme activity in the translational incubation mixture was detected by measuring NADH oxidation in the presence of alpha-ketoglutarate and ammonia as a decrease in absorbency 340-442 nm in a dual wavelength Aminco DW-2 spectrophotometer.

摘要

通过蔗糖密度梯度离心法从小牛肝脏多聚核糖体聚（A）-mRNA组分中部分纯化了谷氨酸脱氢酶的特异性信使RNA。在翻译孵育混合物中的酶活性通过在双波长Aminco DW-2分光光度计中在α-酮戊二酸和氨存在下测量NADH氧化来检测，表现为340-442nm吸光度的下降。

相似文献

1
Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe.使用分光光度探针鉴定谷氨酸脱氢酶的信使核糖核酸
Mol Biol Rep. 1986;11(3):131-5. doi: 10.1007/BF00419732.
2
Translation of glutamate dehydrogenase mRNA in a reticulocyte cell-free system.
Physiol Chem Phys. 1979;11(1):71-5.
3
Translation of mRNA for glutamate dehydrogenase and spectrophotometric procedure to follow the enzyme biosynthesis.谷氨酸脱氢酶信使核糖核酸的翻译及跟踪该酶生物合成的分光光度法。
Arch Int Physiol Biochim. 1977 Aug;85(3):583-8. doi: 10.3109/13813457709069874.
4
Studies on a rat liver subcellular poly A-rich RNA fraction with glutamate dehydrogenase template activity.关于具有谷氨酸脱氢酶模板活性的大鼠肝脏亚细胞富含多聚腺苷酸RNA组分的研究。
Physiol Chem Phys. 1977;9(3):205-15.
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Isolation and characterization of poly(adenylic acid)-containing messenger ribonucleic acid from rat liver polysomes.从大鼠肝脏多核糖体中分离并鉴定含聚腺苷酸的信使核糖核酸
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Purification of messenger RNA coding for glycine methyltransferase from rat liver.从大鼠肝脏中纯化编码甘氨酸甲基转移酶的信使核糖核酸
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Cell-free synthesis and processing of a large precursor of glutamate dehydrogenase of rat liver.大鼠肝脏谷氨酸脱氢酶大前体的无细胞合成与加工
Biochem Biophys Res Commun. 1982 Aug;107(3):1028-36. doi: 10.1016/0006-291x(82)90625-8.

本文引用的文献

1
Use of antibodies for the isolation of biologically pure messenger ribonucleic acid from fully functional eukaryotic cells.使用抗体从功能完整的真核细胞中分离生物纯信使核糖核酸。
Biochemistry. 1974 Apr 23;13(9):1875-85. doi: 10.1021/bi00706a016.
2
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.通过寡聚胸苷酸纤维素柱层析法纯化具有生物活性的珠蛋白信使核糖核酸。
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12. doi: 10.1073/pnas.69.6.1408.
3
Messenger RNA in undeveloped and developing Artemia salina embryos.
未发育和发育中的卤虫胚胎中的信使核糖核酸
Biochem Biophys Res Commun. 1976 Mar 8;69(1):181-9. doi: 10.1016/s0006-291x(76)80289-6.
4
An efficient mRNA-dependent translation system from reticulocyte lysates.一种来自网织红细胞裂解物的高效mRNA依赖性翻译系统。
Eur J Biochem. 1976 Aug 1;67(1):247-56. doi: 10.1111/j.1432-1033.1976.tb10656.x.
5
Studies on a rat liver subcellular poly A-rich RNA fraction with glutamate dehydrogenase template activity.关于具有谷氨酸脱氢酶模板活性的大鼠肝脏亚细胞富含多聚腺苷酸RNA组分的研究。
Physiol Chem Phys. 1977;9(3):205-15.
6
Glutamate dehydrogenase activity in subcellular fractions of mouse fibrosarcoma.
Mol Biol Rep. 1979 Dec 31;5(4):251-2. doi: 10.1007/BF00782898.
7
Translation of glutamate dehydrogenase mRNA in a reticulocyte cell-free system.
Physiol Chem Phys. 1979;11(1):71-5.
8
Hormonal induction of glutamate dehydrogenase in rat liver.
Experientia. 1976 May 15;32(5):653-5. doi: 10.1007/BF01990217.
9
Spectra of glutamate dehydrogenase with diethylstilbestrol.己烯雌酚作用下谷氨酸脱氢酶的光谱
Arch Int Physiol Biochim. 1978 Feb;86(1):11-24. doi: 10.3109/13813457809069880.