使用分光光度探针鉴定谷氨酸脱氢酶的信使核糖核酸
Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe.
作者信息
Hillar M, Orudiakomo S
出版信息
Mol Biol Rep. 1986;11(3):131-5. doi: 10.1007/BF00419732.
PMID:2876375
Abstract
Specific messenger RNA for glutamate dehydrogenase was partially purified from a calf liver polysomal poly(A)-mRNA fraction by sucrose density gradient centrifugation. Enzyme activity in the translational incubation mixture was detected by measuring NADH oxidation in the presence of alpha-ketoglutarate and ammonia as a decrease in absorbency 340-442 nm in a dual wavelength Aminco DW-2 spectrophotometer.
摘要
通过蔗糖密度梯度离心法从小牛肝脏多聚核糖体聚（A）-mRNA组分中部分纯化了谷氨酸脱氢酶的特异性信使RNA。在翻译孵育混合物中的酶活性通过在双波长Aminco DW-2分光光度计中在α-酮戊二酸和氨存在下测量NADH氧化来检测，表现为340-442nm吸光度的下降。
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