通过RPC - 5柱上高压柱色谱法对DNA限制片段进行制备性分级分离。 - Suppr

Landy A, Foeller C, Reszelbach R, Dudock B

Nucleic Acids Res. 1976 Oct;3(10):2575-92. doi: 10.1093/nar/3.10.2575.

High pressure liquid chromatography on the RPC-5 reversed-phase ion exchange system has been shown to have several potential applications as an initial high capacity step in the isolation of specific DNA restriction fragments. The fractionation of the Hinc II digest of lambda DNA, which contains 35 fragments with "flush ends" ranging in size from 3 x 10(6) to 7 x 10(4) daltons, has been used as a model system. Under certain conditions there are some restriction fragments whose elution relative to other fragments is different on RPC-5 chromatography than it is on gel electrophoresis. In some special circumstances it is possible to obtain satisfactory yields (60-70%) of a pure restriction fragment after a single passage through an RPC-5 column.

已证明，在RPC - 5反相离子交换系统上进行的高压液相色谱法，作为分离特定DNA限制片段的初始高容量步骤，有几个潜在应用。λ噬菌体DNA的Hinc II酶切片段包含35个平端片段，大小从3×10⁶到7×10⁴道尔顿，其分级分离已用作模型系统。在某些条件下，有些限制片段在RPC - 5色谱上相对于其他片段的洗脱情况与在凝胶电泳上不同。在某些特殊情况下，单个限制片段单次通过RPC - 5柱后，有可能获得令人满意的产率（60 - 70%）。