Gough G R, Nadeau J G, Gilham P T, Singleton C K, Weith H L
Nucleic Acids Symp Ser. 1980(7):99-102.
Suitably protected ribonucleotide and ribonucleoside derivatives have been employed as versatile intermediates in both oligoribo- and oligodeoxyribonucleotide synthesis by the phosphotriester method. Thus, the barium salt of 5'-O-dimethoxytrityl-2'-O-(o-nitrobenzyl) uridine-3' p-chlorophenyl-phosphate was used to prepare U-U-U as one example of a procedure that has general applicability in the construction of oligoribonucleotides. In addition, ribonucleosides like N4,O2',O3'-tribenzoylcytidine, and ribonucleotides such as the 2',3'-O-bis(dimethoxytrityl) and 2',3'-O-methoxymethylidene derivatives of uridine-5' p-chlorophenyl phosphate, provide a convenient means of introducing 3'- and/or 5'-terminal phosphate residues into oligonucleotides at the phosphotriester level.
适当保护的核糖核苷酸和核糖核苷衍生物已被用作磷酸三酯法合成寡核糖核苷酸和寡脱氧核糖核苷酸的通用中间体。因此，5'-O-二甲氧基三苯甲基-2'-O-(邻硝基苄基)尿苷-3'-对氯苯基磷酸钡盐被用于制备U-U-U，这是一种在寡核糖核苷酸构建中具有普遍适用性的方法的一个例子。此外，像N4,O2',O3'-三苯甲酰基胞苷这样的核糖核苷，以及尿苷-5'-对氯苯基磷酸的2',3'-O-双(二甲氧基三苯甲基)和2',3'-O-甲氧基亚甲基衍生物等核糖核苷酸，为在磷酸三酯水平将3'-和/或5'-末端磷酸残基引入寡核苷酸提供了一种便利的方法。
