Inoue H, Hayase Y, Asaka M, Imura A, Iwai S, Miura K, Ohtsuka E
Nucleic Acids Symp Ser. 1985(16):165-8.
2'-O-Methyl derivatives of common ribonucleosides were prepared from appropriately protected nucleosides via the methylation of the 2'-hydroxyl groups with methyl iodide or diazomethane. These derivatives were converted into 2'-O-methylribonucleotide units and the guanosine derivative was linked to a polymer support. Thus, oligo(2'-O-methylribonucleotides) having the sequence of AmCmUmUmAmCmCmUmGm was synthesized in a stepwise manner by using the phosphotriester method. Thermal stabilities (Tm's) of the hybrid between the 2'-O-methyl ribooligomer and the complementary ribooligomer and of the related hybrids are compared. The synthesis of ribooligomer by a new approach is also described.
常见核糖核苷的2'-O-甲基衍生物是通过用碘甲烷或重氮甲烷对适当保护的核苷的2'-羟基进行甲基化反应制备而成。这些衍生物被转化为2'-O-甲基核糖核苷酸单元，并且鸟苷衍生物被连接到聚合物载体上。因此，通过使用磷酸三酯法逐步合成了具有AmCmUmUmAmCmCmUmGm序列的寡聚（2'-O-甲基核糖核苷酸）。比较了2'-O-甲基核糖寡聚物与互补核糖寡聚物之间杂交体以及相关杂交体的热稳定性（熔解温度）。还描述了通过一种新方法合成核糖寡聚物的过程。
