Kuehl G V, Harkness D R, Skrabut E M, Bechthold D A, Emerson C P, Valeri C R
Vox Sang. 1981 Apr;40(4):260-72. doi: 10.1111/j.1423-0410.1981.tb00704.x.
The intracellular distribution of radioactivity was studied in normal and sickle erythrocytes labeled with sodium 51Cr chromate. Both types of cells had a higher fraction of 51Cr bound to hemoglobin when labeled in the presence of ACD at a pH of 7.09 than when labeled in the presence of CPD at a pH of 5.96. Citrate at a pH of 5.96 or less entered the red blood cells and decreased the 51Cr binding hemoglobin. Binding of 51Cr to hemoglobin within the red cells was also reduced when the ATP and DPG levels in the red blood cells were elevated. Studies with hemoglobin solution showed that 51Cr binding to hemoglobin was influenced by 2,3-DPG, ATP and citrate.
用51铬酸钠标记正常红细胞和镰状红细胞，研究了放射性物质在细胞内的分布情况。当在pH值为7.09的ACD存在下进行标记时，这两种类型的细胞与血红蛋白结合的51铬的比例高于在pH值为5.96的CPD存在下进行标记时的比例。pH值为5.96或更低的柠檬酸盐进入红细胞并降低了51铬与血红蛋白的结合。当红细胞中的ATP和DPG水平升高时，红细胞内51铬与血红蛋白的结合也会减少。对血红蛋白溶液的研究表明，51铬与血红蛋白的结合受2,3-DPG、ATP和柠檬酸盐的影响。
