Higgins M L, Stadnicki S S, Shaw T J, Leach F R
Tex Rep Biol Med. 1978;36:95-110.
After 70 subcultivations of HeLa cells in the presence of amphetamine, diphenhydramine hydrochloride, caffeine, codeine, or morphine, cells were obtained with increased tolerance to the respective drug. Withdrawal of amphetamine, diphenyhydramine hydrochloride or morphine from cells cultivated 645 days in the presence of the drug yielded cells that had either a decreased growth rate in the absence of drug or superior plating efficiencies with low drug concentration when compared to untreated HeLa cells. These observations would be expected from addicted cells. Cinematographic observations showed cell membrane undulation decreased in the presence of morphine. The membrane undulations of cells tolerant to morphine were less sensitive to that drug.
在含有苯丙胺、盐酸苯海拉明、咖啡因、可待因或吗啡的条件下对HeLa细胞进行70次传代培养后，获得了对相应药物耐受性增强的细胞。在含有药物的条件下培养645天的细胞去除苯丙胺、盐酸苯海拉明或吗啡后，与未处理的HeLa细胞相比，得到的细胞在无药物时生长速率降低，或在低药物浓度下具有更高的平板接种效率。这些观察结果与成瘾细胞的预期相符。电影摄影观察显示，在吗啡存在的情况下细胞膜波动减少。对吗啡耐受的细胞的膜波动对该药物不太敏感。
