Tunbridge L J, Lloyd J V, Penhall R K, Wise A L, Maloney T
Am J Hosp Pharm. 1981 Jul;38(7):1001-4.
The stability of heparin diluted in 0.9% sodium chloride injection and stored in plastic syringes for a three-week period was studied. Heparin activity was assayed by the activated partial thromboplastin time (APTT) method. Heparin sodium (25,000 units/ml) was diluted to 500 units/ml and stored in 50-ml polypropylene syringes. Concentrations were compared in two brands of syringes stored at room temperature in the dark. In another experiment controlled for order-related assay errors, heparin was stored in one brand by syringe at either 0-4 degrees C or room temperature. There was a statistically decrease in heparin activity over three weeks in both syringes and at both 0-4 degrees C and room temperature. However, the overall drop in activity was only about 8%. Analysis of covariance confirmed significant regression with time at both temperatures. An unexpected finding was that heparin at 500 units/ml consistently assayed higher than this value. A study of the effect of glass and plastic showed that when heparin was diluted into either a glass or plastic container, there was significantly less heparin activity in the glass containers within two hours. One possible explantation for this phenomenon is absorption of heparin to glass surfaces. It was concluded that heparin can be stored in polypropylene syringe for up to three weeks without refrigeration. However, once diluted, heparin should not be stored in glass containers.
研究了肝素稀释于0.9%氯化钠注射液中并储存在塑料注射器中三周的稳定性。通过活化部分凝血活酶时间（APTT）法测定肝素活性。将肝素钠（25,000单位/毫升）稀释至500单位/毫升，并储存在50毫升聚丙烯注射器中。比较了两种品牌的注射器在室温黑暗条件下储存时的浓度。在另一项控制与顺序相关的测定误差的实验中，肝素以一种品牌的注射器在0 - 4℃或室温下储存。在两种注射器中以及在0 - 4℃和室温下，肝素活性在三周内均有统计学意义的下降。然而，活性的总体下降仅约8%。协方差分析证实了在两个温度下活性随时间均有显著回归。一个意外发现是，500单位/毫升的肝素测定值始终高于该值。一项关于玻璃和塑料影响的研究表明，当肝素稀释到玻璃或塑料容器中时，两小时内玻璃容器中的肝素活性显著降低。对此现象的一种可能解释是肝素吸附到玻璃表面。得出的结论是，肝素可在聚丙烯注射器中储存长达三周而无需冷藏。然而，一旦稀释，肝素不应储存在玻璃容器中。
