Van Marck E A, Beckers A, Deelder A M, Jacob W, Wery M, Gigase P L
Am J Trop Med Hyg. 1981 Jul;30(4):780-9. doi: 10.4269/ajtmh.1981.30.780.
Two recently isolated stocks of Trypanosoma brucei gambiense of human origin gave rise to a moderate to severe proliferative or membranoproliferative glomerulonephritis in 40 or 44 NMRI and C57BL/6J mice infected for 7-22 weeks. Extensive granular deposits of C3, IgG1 and IgG3 were found in the mesangium, together with smaller quantities of IgG2a, IgG2b, and IgM. No trypanosomal antigen could be detected in the deposits though specific anti-trypanosoma antibodies were found in kidney eluates. By electron microscopy, a conspicuous proliferation of mesangial and endothelial cells was observed and electron-dense deposits were seen in a mesangial and subepithelial localization. With one of these trypanosome stocks, four of seven Wistar rats infected for 9-15 weeks developed morphologically similar glomerular lesions. Four other trypanosome stocks did not evoke renal alterations in 17 other rats infected for 13-56 weeks. Experimental infection in mice or rats appears to be a suitable model for the study of renal disease in chronic African sleeping sickness.
最近从人类身上分离出的两株布氏冈比亚锥虫，在感染7至22周的40只NMRI小鼠和44只C57BL/6J小鼠中引发了中度至重度的增殖性或膜增生性肾小球肾炎。在系膜中发现了大量C3、IgG1和IgG3的颗粒状沉积物，同时还有少量的IgG2a、IgG2b和IgM。尽管在肾脏洗脱物中发现了特异性抗锥虫抗体，但在沉积物中未检测到锥虫抗原。通过电子显微镜观察，可见系膜细胞和内皮细胞明显增殖，在系膜和上皮下定位处可见电子致密沉积物。用其中一株锥虫感染9至15周的7只Wistar大鼠中，有4只出现了形态相似的肾小球病变。另外4株锥虫在感染13至56周的17只其他大鼠中未引起肾脏改变。小鼠或大鼠的实验性感染似乎是研究慢性非洲昏睡病肾病的合适模型。
