Lindsley H B, Nagle R B, Stechschulte D J
Am J Trop Med Hyg. 1978 Sep;27(5):864-72. doi: 10.4269/ajtmh.1978.27.864.
Host immunologic responses were studied in Sprague-Dawley rats infected with a human isolate of Trypanosoma rhodesiense. Retro-orbital sinus bleedings were obtained on days 0 and 14 and at 21 or 28 days. Infected and control rats were sacrificed on day 21 or 28 of the infection. Mild glomerulitis, as assessed by increased cellcularity and/or widening of the mesangial region, was apparent in 6 of 7 infected animals. Finely granular deposits of IgM and IgGl were found in most glomeruli with less prominent deposits of IgGa and IgA. Focal cortical mononuclear interstitial infiltrates were present in 2 of 7 rats. Subepithelial or mesangial electron dense deposits were present in the glomeruli of infected rats. Hypocomplementemia was present by day 21 and involved both the classical and alternative pathways of complement activation. Antibodies to native DNA and single-stranded RNA were present by day 21. These studies indicate the feasibility of studying trypanosomal glomerulonephritis in rats and will provide a convenient model for detailed immunologic and morphologic investigation of host immune responses in African trypanosomiasis.
对感染了罗得西亚锥虫人分离株的斯普拉格-道利大鼠的宿主免疫反应进行了研究。在第0天和第14天以及第21天或第28天进行眶后窦采血。在感染的第21天或第28天处死感染大鼠和对照大鼠。在7只感染动物中的6只中，通过细胞增多和/或系膜区增宽评估发现轻度肾小球肾炎。在大多数肾小球中发现IgM和IgG1的细颗粒沉积物，IgG2a和IgA的沉积物不那么明显。7只大鼠中有2只出现局灶性皮质单核细胞间质浸润。感染大鼠的肾小球中存在上皮下或系膜电子致密沉积物。到第21天时出现补体低下，涉及补体激活的经典途径和替代途径。到第21天时出现针对天然DNA和单链RNA的抗体。这些研究表明在大鼠中研究锥虫性肾小球肾炎的可行性，并将为详细免疫和形态学研究非洲锥虫病宿主免疫反应提供一个便利的模型。
