Walsh P R, Wang M C, Gitterman M L
Ann Clin Lab Sci. 1981 Mar-Apr;11(2):138-45.
The analytical evaluation is described of a [125I]-aldosterone radioimmunoassay method for measuring aldosterone in human plasma, which requires no chromatographic purification before quantification by radioimmunoassay but does require organic solvent extraction. Rabbit anti-aldosterone serum is used, generated against aldosterone-3-oxime coupled to thyroglobulin. The antibody demonstrates negligible cross reactions with structurally-related steroids. The proposed method uses [125I]-labeled aldosterone as the radioactive ligand. The recovery of D-aldosterone added to human plasma averaged 98 percent. The parallelism and precision of the method are excellent. Seventy specimens were assayed by the proposed method and a [3H]-aldosterone reference procedure, which includes LH-20 column chromatographic purification prior to the radioimmunoassay step (y = 1.03X - 3.0; r = 0.99; p less than 0.01). The [3H]-aldosterone reference procedure uses an anti-aldosterone serum prepared against aldosterone-3-oxime coupled to rabbit serum albumin.
本文描述了一种用于测定人血浆中醛固酮的[125I]-醛固酮放射免疫分析方法的分析评估。该方法在通过放射免疫分析进行定量之前无需色谱纯化，但需要有机溶剂萃取。使用针对与甲状腺球蛋白偶联的醛固酮-3-肟产生的兔抗醛固酮血清。该抗体与结构相关的类固醇的交叉反应可忽略不计。所提出的方法使用[125I]-标记的醛固酮作为放射性配体。添加到人血浆中的D-醛固酮的回收率平均为98%。该方法的平行性和精密度极佳。通过所提出的方法和[3H]-醛固酮参考程序对70个样本进行了分析，[3H]-醛固酮参考程序在放射免疫分析步骤之前包括LH-20柱色谱纯化（y = 1.03X - 3.0；r = 0.99；p小于0.01）。[3H]-醛固酮参考程序使用针对与兔血清白蛋白偶联的醛固酮-3-肟制备的抗醛固酮血清。
