Orrison L H, Cherry W B, Fliermans C B, Dees S B, McDougal L K, Dodd D J
Appl Environ Microbiol. 1981 Jul;42(1):109-15. doi: 10.1128/aem.42.1.109-115.1981.
Thirty-eight cultures of Legionella pneumophila isolated from surface waters were characterized by their morphological, tinctorial, biochemical, and serological properties and by their ability to produce disease in guinea pigs. Their susceptibility to antimicrobial agents also was tested. When they were compared with clinical isolates, no important differences were found between cultures from the two sources. Sodium hippurate hydrolysis, gelatin liquefaction, pigment formation, and beta-lactamase and alkaline phosphatase activity were useful in differentiating the four described species of Legionella. Hydrolysis of diacetylfluorescein and the inability to reduce nitrate help to distinguish Legionella species from other gram-negative bacterial rods.
从地表水中分离出的38株嗜肺军团菌菌株,通过其形态学、染色特性、生化特性、血清学特性以及在豚鼠体内引发疾病的能力进行了鉴定。还测试了它们对抗菌剂的敏感性。当将它们与临床分离株进行比较时,发现来自这两种来源的培养物之间没有重要差异。马尿酸钠水解、明胶液化、色素形成以及β-内酰胺酶和碱性磷酸酶活性,有助于区分已描述的四种军团菌。二乙酰荧光素的水解以及无法还原硝酸盐,有助于将军团菌属与其他革兰氏阴性杆菌区分开来。