立克次氏体样生物TATLOCK（1943年）和HEBA（1959年）：表型与嗜肺军团菌和WIGA细菌相似但基因不同的细菌。

The rickettsia-like organisms TATLOCK (1943) and HEBA (1959): bacteria phenotypically similar to but genetically distinct from Legionella pneumophila and the WIGA bacterium.

作者信息

Hébert G A, Moss C W, McDougal L K, Bozeman F M, McKinney R M, Brenner D J

出版信息

Ann Intern Med. 1980 Jan;92(1):45-52. doi: 10.7326/0003-4819-92-1-45.

Abstract

Two "rickettsia-like organisms," TATLOCK and HEBA, isolated from human blood via guinea pigs and embryonated eggs in 1943 and 1959, respectively, have been cultured on artificial media (charcoal yeast extract agar) for the first time and characterized. TATLOCK and HEBA have identical cultural, biochemical, and antigenic characteristics, as well as identical cellular fatty-acid composition and antimicrobial susceptibilities. These two bacteria have most of the cultural and biochemical characteristics of Legionella pneumophilia, and their gas-liquid chromatography cellular fatty-acid profile is similar to that of WIGA, another bacterium similar to L. pneumophila. Direct fluorescent-antibody reagents prepared for HEBA and TATLOCK gave equal high-titered reciprocal staining and were negative on 220 other bacteria, including L. pneumophila. Deoxyribonucleic acid relatedness studies, however, showed that these bacteria are not genetically related to either L. pneumophila or the WIGA bacterium.

摘要

1943年和1959年分别通过豚鼠和胚胎卵从人血中分离出的两种“类立克次氏体生物”，即TATLOCK和HEBA，首次在人工培养基（木炭酵母提取物琼脂）上培养并进行了特性鉴定。TATLOCK和HEBA具有相同的培养、生化和抗原特性，以及相同的细胞脂肪酸组成和抗菌敏感性。这两种细菌具有嗜肺军团菌的大部分培养和生化特性，并且它们的气-液色谱细胞脂肪酸图谱与另一种类似于嗜肺军团菌的细菌WIGA相似。针对HEBA和TATLOCK制备的直接荧光抗体试剂产生了相同的高滴度相互染色，并且对包括嗜肺军团菌在内的其他220种细菌呈阴性。然而，脱氧核糖核酸相关性研究表明，这些细菌在基因上与嗜肺军团菌或WIGA细菌均无关联。