Effect of isoproterenol and propranolol on the mast cell degranulation and on the passive cutaneous anaphylaxis in mice.

Effect of isoproterenol and propranolol on the production of hemocytotropic antibodies and degranulation of peritoneal mast cells from sensitized mice was studied. It was demonstrated that stimulation with large doses of the antigen caused degranulation of peritoneal mast cells and that additional administration of a beta antagonist propranolol failed to increase the degranulation phenomenon. Marked inhibition in degranulation of mast cells was seen in mice sensitized with large and small doses of antigen after isoproterenol. In 2h PCA test in animals immunized with large doses of the antigen a higher activity of PCA reaction illustrating the concentration of IgG1 antibodies was noted. With higher doses of the antigen and propranolol the titers of the antibodies were markedly higher. The inhibiting effect of isoprenaline on the passive cutaneous anaphylaxis was observed only in the 72 h-test, mostly in the group of mice immunized with small doses of the antigen.