Liu X, Feng J, Xu Z R, Wang Y Z, Liu J X
The Key Laboratory of Molecular Animal Nutrition, College of Animal Science, Zhejiang University, Ministry of Education, HangZhou, China.
Clin Exp Allergy. 2008 Feb;38(2):350-6. doi: 10.1111/j.1365-2222.2007.02893.x. Epub 2007 Dec 7.
Soybean protein is used in a number of food products but is also a common cause of food allergy. Soybean glycinin and beta-conglycinin represent up to one-third of protein in the soybean. Many reports have indicated that glycinin and beta-conglycinin have been characterized as major soybean allergens involved in food hypersensitivity.
To investigate oral allergy syndrome and anaphylactic reactions in BALB/c mice caused by soybean glycinin and beta-conglycinin with an intragastric feeding protocol without using an adjuvant.
BALB/c mice were sensitized by gavages with glycinin and beta-conglycinin, and allergen-specific IgE and IgG1 responses were studied by a passive cutaneous anaphylaxis assay. Serum histamine release and blood pressure were measured according to other methods. Epithelium and mast cell dye used the method of light microscopy.
Sensitization with soybean allergens induced high levels of antigen-specific IgE and IgG1 and increased serum histamine in BALB/c mice. Percentiles of intact mast cell of small intestine in mice sensitized with glycinin and beta-conglyinin significantly decreased for 28 days. Degranulation of mast cells and damage of the epithelium in the small intestine of mice sensitized with globulins were observed. The level of blood pressure in sensitized mice reached a minimum at 3 h.
Soybean-specific IgE and IgG1 antibodies increased, with high levels of histamine release, severe degranulation of mast cells and damage of the epithelium of small intestine in mice sensitized with glycinin and beta-conglyinin.
大豆蛋白被用于多种食品中，但也是食物过敏的常见原因。大豆球蛋白和β-伴大豆球蛋白占大豆中蛋白质的三分之一。许多报告表明，大豆球蛋白和β-伴大豆球蛋白已被确定为参与食物过敏反应的主要大豆过敏原。
采用无佐剂的灌胃方案，研究大豆球蛋白和β-伴大豆球蛋白对BALB/c小鼠引起的口腔过敏综合征和过敏反应。
用大豆球蛋白和β-伴大豆球蛋白对BALB/c小鼠进行灌胃致敏，通过被动皮肤过敏试验研究过敏原特异性IgE和IgG1反应。根据其他方法测量血清组胺释放和血压。上皮细胞和肥大细胞染色采用光学显微镜法。
用大豆过敏原致敏可诱导BALB/c小鼠产生高水平的抗原特异性IgE和IgG1，并增加血清组胺。用大豆球蛋白和β-伴大豆球蛋白致敏的小鼠小肠中完整肥大细胞的百分率在28天内显著降低。观察到用球蛋白致敏的小鼠小肠中肥大细胞脱颗粒和上皮损伤。致敏小鼠的血压水平在3小时时降至最低。
用大豆球蛋白和β-伴大豆球蛋白致敏的小鼠中，大豆特异性IgE和IgG1抗体增加，组胺释放水平高，肥大细胞严重脱颗粒，小肠上皮受损。
