Canick J A, Ryan K J
Steroids. 1978 Nov;32(4):499-509. doi: 10.1016/0039-128x(78)90061-2.
The aromatase system associated with the mitochondrial fraction of human term placenta, present at 35--50% the specific activity of the microsomal enzyme, is substantially the same as the microsomal enzyme as determined by the following: 1) The rate of aromatization of androstenedione, 19-nortestosterone, and 16alpha-hydroxytestosterone in mitochondria was a nearly constant proportion of the microsomal rate; 2) Sensitivity to carbon monoxide was the same; 3) The magnitude of cytochrome P-450 Type I spectral interactions with androgen substrates was a constant proportion in mitochondria and microsomes; 4) Sensitivity to an antibody raised against hepatic microsomal NADPH-cytochrome c reductase was the same. When inner and outer mitochondrial membrane subfractions were prepared, the predominant aromatase activity was associated with the outer membrane preparation. This aromatase activity could not be accounted for by microsomal contamination as determined by inosine diphosphatase activity, a microsomal marker. After correction, the rate of aromatization in the outer membrane preparation was almost six times that in the inner membranes and three times that of the whole mitochondrial fraction.
与足月人胎盘线粒体部分相关的芳香化酶系统，其比活性为微粒体酶的35%-50%，通过以下方面测定，该系统与微粒体酶基本相同：1）线粒体中雄烯二酮、19-去甲睾酮和16α-羟基睾酮的芳香化速率与微粒体速率的比例几乎恒定；2）对一氧化碳的敏感性相同；3）线粒体和微粒体中细胞色素P-450 I型与雄激素底物的光谱相互作用强度比例恒定；4）对针对肝微粒体NADPH-细胞色素c还原酶产生的抗体的敏感性相同。当制备线粒体内外膜亚组分时，主要的芳香化酶活性与外膜制备物相关。由肌苷二磷酸酶活性（一种微粒体标志物）测定，该芳香化酶活性不能用微粒体污染来解释。校正后，外膜制备物中的芳香化速率几乎是内膜中的六倍，是整个线粒体部分的三倍。
