Barrow D A, Lentz B R
Biochim Biophys Acta. 1981 Jul 6;645(1):17-23. doi: 10.1016/0005-2736(81)90506-x.
We have determined by means of a standard spectrophotometric assay that lipid oxidation occurred at a significant rate in large, multilamellar vesicles containing egg phosphatidylcholine under normal experimental conditions. We have also observed that the fluorescence intensity of the vesicle-associated probe, 1,6-diphenyl-1,3,5-hexatriene, decreased with time in vesicles containing such oxidizing lipids. The spectrophotometric data utilized to monitor lipid oxidation were found to fit an apparent first-order kinetic model. The loss of diphenylhexatriene fluorescence intensity in oxidizing liposomes was analyzed in terms of a first-order event superimposed (and thus presumably dependent) upon the ongoing formation of oxidized lipid. These and other data were used to conclude that the oxidation-induced loss of diphenylhexatriene fluorescence intensity was due to chemical modification of the fluorophore rather than to excited-state quenching or ground-state complex formation. Finally, the loss of fluorescence intensity in oxidizable membranes was found to alter drastically the 'microviscosity' parameter as derived from diphenylhexatriene fluorescence anisotropy and relative intensity measurements.
我们通过标准分光光度法测定发现，在正常实验条件下，含有鸡蛋磷脂酰胆碱的大型多层囊泡中脂质氧化以显著速率发生。我们还观察到，在含有此类氧化脂质的囊泡中，与囊泡相关的探针1,6 - 二苯基 - 1,3,5 - 己三烯的荧光强度随时间降低。用于监测脂质氧化的分光光度数据被发现符合表观一级动力学模型。氧化脂质体中双苯基己三烯荧光强度的损失根据叠加在正在进行的氧化脂质形成之上（因此可能依赖于此）的一级事件进行分析。这些数据以及其他数据被用于得出结论，即氧化诱导的双苯基己三烯荧光强度损失是由于荧光团的化学修饰，而非激发态猝灭或基态复合物形成。最后，发现可氧化膜中荧光强度的损失极大地改变了由双苯基己三烯荧光各向异性和相对强度测量得出的“微粘度”参数。
