Dale R E, Chen L A, Brand L
J Biol Chem. 1977 Nov 10;252(21):7500-10.
The rotational relaxation of the widely used "microviscosity" probe, 1,6-diphenyl-1,3,5-hexatriene, was examined by the technique of nanosecond time-resolved fluorescence depolarization. The decays of the emission anisotropy were determined at five temperatures in the range 3-31 degrees both in a reference paraffin oil and in sonicated egg lecithin vesicles. These decays were complex in both media. Marked qualitative as well as quantitative differences were observed in the rotational behavior of the probe in the complex bilayer medium as opposed to the homogeneous reference solvent. The results are discussed in relation to the structure of the hydrophobic bilayer membrane interior and the concept of its "microviscosity".
采用纳秒时间分辨荧光去极化技术研究了广泛使用的“微粘度”探针1,6 - 二苯基 - 1,3,5 - 己三烯的旋转弛豫。在3至31度范围内的五个温度下，分别在参比石蜡油和经超声处理的卵磷脂囊泡中测定了发射各向异性的衰减。在两种介质中，这些衰减都是复杂的。与均匀的参比溶剂相比，在复合双层介质中观察到探针的旋转行为存在明显的定性和定量差异。结合疏水双层膜内部结构及其“微粘度”概念对结果进行了讨论。
