[冷冻溶液的温度和组成对2,3-二甲基-1,4-萘醌-1-二甲基磷酸酯过氧化物酶氧化速率的影响]

[Effect of temperature and composition of frozen solutions on the rate of peroxidase oxidation of 2,3-dimethyl-1,4-naphthoquinol-1-dimethylphosphate].

作者信息

Sergeev G B, Sergeev B M, Kalnina I A, Nekrasova G N, Batiuk V A

出版信息

Biokhimiia. 1981 Nov;46(11):2089-91.

Abstract

The horseradish peroxidase-catalyzed oxidation of 2.3-dimethyl-1.4-naphthoquinol-1-dimethylphosphate by hydrogen peroxide was investigated within the temperature range of +20 divided by -35 degrees. The increase in the concentration of the buffer solution accelerated the reaction in liquid solutions at 0 degrees and 20 degrees. The unusual shapes of the curves of temperature dependences of the reaction rate in frozen solutions of different composition can probably be due to temperature variations in some physico-chemical parameters of the system under study.

摘要

在+20至-35摄氏度的温度范围内，研究了辣根过氧化物酶催化过氧化氢氧化2,3-二甲基-1,4-萘醌-1-二甲基磷酸酯的反应。缓冲溶液浓度的增加加速了0摄氏度和20摄氏度下液体溶液中的反应。不同组成的冷冻溶液中反应速率的温度依赖性曲线形状异常，可能是由于所研究系统的一些物理化学参数随温度变化所致。

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