Pepper M D, Learoyd P A, Rajah S M
Transfusion. 1978 Nov-Dec;18(6):756-60. doi: 10.1046/j.1537-2995.1978.18679077963.x.
Some of the variables affecting factor VIII during storage of plasma at 4 C prior to preparing factor VIII concentrates and cryoprecipitates were investigated. No significant difference in factor VIII levels could be demonstrated between whole blood, platelet-rich plasma or plasma depleted of platelets when stored at 4 C. However, in frozen plasma platelets were shown to have a deleterious effect on factor VIII. No significant difference in factor VIII levels was found in whole blood or plasma stored at 4 C for 6 hours as compared with blood or plasma stored for 18 hours (p = greater than 0.1). There was, however, a highly significant difference (p = less than 0.001) between plasma and whole blood stored at 4 C for 4 hours as compared with storage for 18 hours. There was a significant correlation (correlation coefficient 0.714) between factor VIII levels in the starting material and the factor VIII recovered in cryoprecipitates, but in a small pilot study no correlation between factor VIII levels in the starting material and that recovered in freeze-dried concentrates, could be found. There was no significant difference in factor VIII levels between the Group O and Group A donations used in this study (p = greater than 0.1), and no correlation was found between the drop in pH during storage and the factor VIII decay pattern.
在制备凝血因子 VIII 浓缩物和冷沉淀之前，对 4℃保存血浆期间影响凝血因子 VIII 的一些变量进行了研究。全血、富含血小板血浆或血小板贫乏血浆在 4℃保存时，凝血因子 VIII 水平无显著差异。然而，冷冻血浆中的血小板对凝血因子 VIII 有有害影响。4℃保存 6 小时的全血或血浆与保存 18 小时的血液或血浆相比，凝血因子 VIII 水平无显著差异（p＞0.1）。但是，4℃保存 4 小时的血浆和全血与保存 18 小时相比，存在高度显著差异（p＜0.001）。起始材料中的凝血因子 VIII 水平与冷沉淀中回收的凝血因子 VIII 之间存在显著相关性（相关系数 0.714），但在一项小型初步研究中，未发现起始材料中的凝血因子 VIII 水平与冻干浓缩物中回收的凝血因子 VIII 之间存在相关性。本研究中使用的 O 型和 A 型献血的凝血因子 VIII 水平无显著差异（p＞0.1），并且未发现保存期间 pH 值下降与凝血因子 VIII 衰减模式之间存在相关性。
