Smith J K, Snape T J, Haddon M E, Gunson H H, Edwards R
Transfusion. 1978 Sep-Oct;18(5):530-7. doi: 10.1046/j.1537-2995.1978.18579036380.x.
Minimally destructive methods were sought to assess the factor VIII content of fresh frozen plasma intended for large-scale fractionation and stored in five-liter polyethylene packs after pooling approximately 23 plasma donations. Although factor VII distribution in the frozen pack was not perfectly homogenous, a central "core" through the frozen pack gave a representative sampel of the entire contents of the pack. Coring was compared with other methods of pack sampling before large-scale cryoprecipitation. The survival of factor VIII was studied in three grades of stored plasma which had been separated and frozen within 4 hours, 8 hours, and 18 hours of blood donation. Plasma frozen after overnight storage of blood is a satisfacory starting material for the preparation of factor VIII and factor IX concentrates.
为了评估用于大规模分馏且在汇集约23份血浆捐献后储存在5升聚乙烯包装中的新鲜冰冻血浆的凝血因子VIII含量，人们寻求了微创方法。尽管冷冻包装中凝血因子VII的分布并非完全均匀，但通过冷冻包装的中心“核心”可获得代表该包装全部内容物的样本。在大规模冷沉淀之前，将取芯法与其他包装取样方法进行了比较。研究了在献血后4小时、8小时和18小时内分离并冷冻的三个等级储存血浆中凝血因子VIII的存活率。过夜储存血液后冷冻的血浆是制备凝血因子VIII和凝血因子IX浓缩物的理想起始材料。
