Suppr超能文献

通过平衡超速离心法对非理想单体N-聚体、等键反应和II型不确定自缔合体系进行图形分析。

Graphical analysis of nonideal monomer N-mer, isodesmic, and type II indefinite self-associating systems by equilibrium ultracentrifugation.

作者信息

Stafford W F

出版信息

Biophys J. 1980 Jan;29(1):149-66. doi: 10.1016/S0006-3495(80)85122-8.

DOI:10.1016/S0006-3495(80)85122-8
PMID:7260244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1328666/
Abstract

A graphical procedure is described by which one can obtain in principle the monomer molecular weight, stoichiometry, equilibrium constant, and second virial coefficient of nonideal monomer N-mer, isodesmic, and type II indefinite self-associating systems. In addition, a method is presented for obtaining both the equilibrium constant and the second virial coefficient from the maximum in a plot of apparent molecular weight vs. concentration if the monomer molecular weight and stoichiometry are known. The usefulness and limitations of the methods are discussed, as well as the quality and range of data required for determination of the relevant parameters. The techniques described are applicable to analysis of self-associating systems by osmotic pressure and light scattering, as well as equilibrium ultracentrifugation measurements.

摘要

本文描述了一种图解方法，原则上可用于获得非理想单体N聚体、等键反应和II型不确定自缔合体系的单体分子量、化学计量比、平衡常数和第二维里系数。此外，如果已知单体分子量和化学计量比，还提出了一种从表观分子量与浓度关系图中的最大值获取平衡常数和第二维里系数的方法。文中讨论了这些方法的实用性和局限性，以及确定相关参数所需数据的质量和范围。所描述的技术适用于通过渗透压、光散射以及平衡超速离心测量对自缔合体系进行分析。

相似文献

1
Graphical analysis of nonideal monomer N-mer, isodesmic, and type II indefinite self-associating systems by equilibrium ultracentrifugation.通过平衡超速离心法对非理想单体N-聚体、等键反应和II型不确定自缔合体系进行图形分析。
Biophys J. 1980 Jan;29(1):149-66. doi: 10.1016/S0006-3495(80)85122-8.
2
Self-association mode of a flavoenzyme D-amino acid oxidase from hog kidney. II. Stoichiometry of holoenzyme association and energetics of subunit association.猪肾黄素酶D-氨基酸氧化酶的自缔合模式。II. 全酶缔合的化学计量和亚基缔合的能量学
J Biol Chem. 1985 Oct 15;260(23):12615-21.
3
Virial expansions for ideal self-associating systems.理想自缔合体系的维里展开式。
Biophys J. 1972 Oct;12(10):1359-65. doi: 10.1016/S0006-3495(72)86167-8.
4
Characterization of macromolecular heterogeneity by equilibrium sedimentation techniques.通过平衡沉降技术对大分子异质性进行表征。
Biophys Chem. 2004 Mar 1;108(1-3):141-63. doi: 10.1016/j.bpc.2003.10.015.
5
Equations for the equilibrium constants of nonideal systems derived from the multinomial theroy using the z-average molecular weight.
Biochemistry. 1972 Mar 14;11(6):1120-1. doi: 10.1021/bi00756a028.
6
Nonideal associating systems. I. Documentation of a new method for determining the parameters from sedimentation equilibrium data.非理想缔合体系。I. 一种从沉降平衡数据确定参数的新方法的文献记录。
J Biol Chem. 1972 Jan 25;247(2):427-39.
7
Equilibrium centrifugation of nonideal systems. Molecular weight moments for removing the effects of nonideality.
Biochemistry. 1972 Aug 1;11(16):2925-34. doi: 10.1021/bi00766a001.
8
Simulation studies of self-associating systems; discrimination between specific and isodesmic associations.自缔合体系的模拟研究；特定缔合与等键缔合之间的区分
Biophys Chem. 1976 Jul;5(1-2):171-83. doi: 10.1016/0301-4622(76)80033-6.
9
Sedimentation equilibrium in reacting systems. 3. Evaluation of the number average (Mn(c)) molecular weight, equilibrium constants, and nonideal effects.
Biochemistry. 1965 Aug;4(8):1646-54. doi: 10.1021/bi00884a029.
10
A comparison of weight average and direct boundary fitting of sedimentation velocity data for indefinite polymerizing systems.无限聚合体系沉降速度数据的重均法与直接边界拟合的比较
Biophys Chem. 2004 Mar 1;108(1-3):215-30. doi: 10.1016/j.bpc.2003.10.029.

引用本文的文献

1
Analysis of nonideality: insights from high concentration simulations of sedimentation velocity data.非理想性分析：来自沉降速度数据高浓度模拟的见解。
Eur Biophys J. 2020 Dec;49(8):687-700. doi: 10.1007/s00249-020-01474-5. Epub 2020 Nov 6.
2
Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.分析超速离心法：沉降速度与沉降平衡
Methods Cell Biol. 2008;84:143-79. doi: 10.1016/S0091-679X(07)84006-4.
3
Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques.采用非线性最小二乘法技术对分析型超速离心机的数据进行分析。
Biophys J. 1981 Dec;36(3):575-88. doi: 10.1016/S0006-3495(81)84753-4.

本文引用的文献

1
PHYSICAL STUDIES OF MURAMIDASE (LYSOZYME). II. PH-DEPENDENT DIMERIZATION.溶菌酶的物理研究。II. 依赖pH的二聚化作用
J Biol Chem. 1964 Aug;239:2516-24.
2
EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.稀溶液的平衡超速离心法
Biochemistry. 1964 Mar;3:297-317. doi: 10.1021/bi00891a003.
3
A sedimentation equilibrium study of the association of purine in aqueous solutions.嘌呤在水溶液中缔合作用的沉降平衡研究。
Biochemistry. 1967 Jul;6(7):2189-94. doi: 10.1021/bi00859a041.
4
A study of the combined sedimentation and chemical equilibrium of beta-lactoglobulin B in acid solution.
Biochemistry. 1968 Jan;7(1):67-78. doi: 10.1021/bi00841a010.
5
Self-association of muramidase (lysozyme) in solution at 25 degrees, pH 7.0, and I = 0.20.在25摄氏度、pH值为7.0且离子强度I = 0.20的溶液中，溶菌酶的自我缔合。
Biochemistry. 1970 Oct 27;9(22):4260-7. doi: 10.1021/bi00824a004.
6
Self-association in the myosin system at high ionic strength. II. Evidence for the presence of a monomer--dimer equilibrium.肌球蛋白系统在高离子强度下的自缔合。II. 单体 - 二聚体平衡存在的证据。
Biochemistry. 1970 Feb 17;9(4):894-908. doi: 10.1021/bi00806a026.
7
Exponential analysis of concentration or concentration difference data for discrete molecular weight distributions in sedimentation equilibrium.沉降平衡中离散分子量分布的浓度或浓度差数据的指数分析。
Biochemistry. 1970 Jan 20;9(2):435-45. doi: 10.1021/bi00804a035.
8
Sedimentation equilibrium of low-molecular-weight associating solutes.
Ann N Y Acad Sci. 1969 Nov 7;164(1):279-93. doi: 10.1111/j.1749-6632.1969.tb14044.x.
9
Studies of self-associating systems by equilibrium ultracentrifugation.通过平衡超速离心法对自缔合体系的研究。
Ann N Y Acad Sci. 1969 Nov 7;164(1):245-78. doi: 10.1111/j.1749-6632.1969.tb14043.x.
10
Equilibrium centrifugation of nonideal systems. The Donnan effect in self-associating systems.非理想体系的平衡离心法。自缔合体系中的唐南效应。
Biochemistry. 1971 Aug 17;10(17):3241-9. doi: 10.1021/bi00793a013.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验