红细胞快速混合实验中的流体动力学和扩散因素
Hydrodynamic and diffusion considerations of rapid-mix experiments with red blood cells.
作者信息
Rice S A
出版信息
Biophys J. 1980 Jan;29(1):65-77. doi: 10.1016/S0006-3495(80)85118-6.
Abstract
From studies of the oxygenation rate of red blood cells (RBC) using rapid-mix techniques, it has been suggested that RBC are surrounded by a stagnant layer of water that does not (or cannot) mix with the rest of the water. A consideration of the appropriate hydrodynamics and convective diffusion rates shows that a mixer can reduce the resolution time to approximately 1 ms (or possibly less) and give a diffusion layer around the TBC that is approximately 1 micron thick. In stopped flow equipment it expands to approximately 4 micron over approximately 10 ms, whereas in continuous flow work the diffusion layers expands slightly less rapidly and less far. Thus the rate of oxygenation of TBC should be slower when measured by stopped flow techniques than by continuous flow apparatus for which the rate will depend weakly on the Reynolds number of the flow in the interrogation tube.
摘要
通过使用快速混合技术对红细胞（RBC）的氧合速率进行研究，有人提出红细胞被一层不（或不能）与其余水混合的停滞水层包围。对适当的流体动力学和对流扩散速率的考虑表明，混合器可以将分辨时间缩短至约1毫秒（或可能更短），并在TBC周围形成约1微米厚的扩散层。在停流设备中，它在约10毫秒内扩展到约4微米，而在连续流工作中，扩散层扩展得稍慢且扩展距离稍短。因此，当用停流技术测量时，TBC的氧合速率应比用连续流设备测量时慢，连续流设备的速率将微弱地取决于询问管中流动的雷诺数。
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