Kutchai H
Respir Physiol. 1975 Jan;23(1):121-32. doi: 10.1016/0034-5687(75)90076-6.
The rate at which red blood cells take up O2 or CO as measured in a rapid reaction apparatus is considerably less than predicted from solution of the equations for diffusion and chemical reaction in a layer of hemoglobin solution about the same thickness as the red cell. Nicolson and Roughton (1951) showed that this discrepancy could be accounted for by postulating that the red cell membrane is an important barrier to gas uptake. Sinha (1969) measured the rate of O2 uptake by single red cells located near a gas-plasma interface. The equations for diffusion and chemical reaction of O2 in a membraneless layer of hemoglobin solution for conditions that correspond to Sinha's experiments are solved. The calculated time course of O2 uptake fits the experimental data sufficiently well to suggest that the resistance of the red cell membrane to O2 diffusion is not an important limiting factor. Also analyzed in this way is the data of Carlson and Comroe (1958). The author finds that calculations predict a faster rate of CO uptake by biconcave disc shaped red cells than was observed experimentally, but that calculations for sphered red cells agree well enough with experimental data that membrane CO permeability may not be primary in limiting CO uptake by spherocytes.
在快速反应装置中所测得的红细胞摄取氧气或一氧化碳的速率，比根据在与红细胞厚度大致相同的一层血红蛋白溶液中扩散和化学反应方程式的解所预测的速率要低得多。尼科尔森和劳顿（1951年）表明，通过假定红细胞膜是气体摄取的重要屏障，可以解释这种差异。辛哈（1969年）测量了位于气 - 血浆界面附近的单个红细胞摄取氧气的速率。针对与辛哈实验条件相对应的无膜血红蛋白溶液层中氧气的扩散和化学反应方程式进行了求解。计算得出的氧气摄取时间进程与实验数据拟合得足够好，表明红细胞膜对氧气扩散的阻力不是一个重要的限制因素。卡尔森和康罗伊（1958年）的数据也以这种方式进行了分析。作者发现，计算预测双凹圆盘形红细胞摄取一氧化碳的速率比实验观察到的要快，但球形红细胞的计算结果与实验数据吻合得足够好，以至于膜对一氧化碳的通透性可能不是限制球形红细胞摄取一氧化碳的主要因素。
