77K 时视紫红质的光转化。量子效率的估算。
The photoconversion of lumirhodopsin at 77 degrees K. Estimation of the quantum efficiency.
作者信息
Becher B
出版信息
Biophys J. 1980 Apr;30(1):1-7. doi: 10.1016/S0006-3495(80)85072-7.
Evidence is presented that lumirhodopsin (containing all-trans retinal) is not directly photoconverted to bathorhodopsin (all-trans) at 77 degrees K as previously suggested (Yoshizawa and Wald. 1963. Nature (Lond.) 197:1279-1286). Rather, lumirhodopsin is converted to a new species, L' (11-cis and/or 9-cis retinal) which, on warming to room temperature, is indistinguishable from rhodopsin or isorhodopsin. The quantum efficiency for the conversion of lumirhodopsin to L' is estimated to be 0.5 +/- 0.1. This value is significantly higher than that of other all-trans to cis conversions for bovine rhodopsin intermediates, indicating that the opsin conformation has a significant effect on a pigment's quantum efficiency.
有证据表明，视紫红质（含有全反式视黄醛）在77K时并不会如之前所认为的那样（吉泽和沃尔德，1963年，《自然》（伦敦）197:1279 - 1286）直接光转化为视紫红质（全反式）。相反，视紫红质会转化为一个新的物种L'（11 - 顺式和/或9 - 顺式视黄醛），当升温至室温时，它与视紫红质或异视紫红质无法区分。视紫红质转化为L'的量子效率估计为0.5±0.1。该值显著高于牛视紫红质中间体其他全反式到顺式转化的值，表明视蛋白构象对色素的量子效率有显著影响。
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