Bhatnagar R, Christian R G, Nakano T, Aherne F X, Thompson J R
Can J Comp Med. 1981 Apr;45(2):188-95.
Age related changes and osteochondrosis in swine were studied using light microscopy and electron microscopy in articular cartilage and light microscopy and epiphyseal cartilage of swine from three days to 30 weeks of age. Thickness, cellularity and vascularity of both the epiphyseal and articular cartilage, decreased as the swine aged. Osteochondrotic changes included formation of "plugs" of cartilage indicating localized failure of ossification and separation and space formation in epiphyseal cartilage. Eosinophilic streaks and space formation in epiphyseal cartilage was observed in relation to epiphyseal separation. Electron microscopy showed a continuous fibrillar layer on the surface of the cartilage corresponding to the lamina splendens of light microscopy. This layer increased in the thickness and showed accumulation of amorphous material between the fibrils with aging. In the matrix, the orientation and distribution of the collagen fibers changed with growth and thicker fibers with clear sub banding were more common in older age groups. Also, necrotic cells, glycogen containing bodies and cellular debris were noticed in the matrix of normal cartilage in old animals. Chondrocytes in the younger cartilage showed accumulation of organelles responsible for protein synthesis; while Golgi bodies, vesicles, lysosomes, well developed foot processes and other inclusions were noticed in older cartilage. Cartilage erosions had a clumped and disrupted lamina splendens on the surface and electron lucent patches in the ground substances of the matrix and chondrocyte cytoplasm.
利用光学显微镜和电子显微镜，对3日龄至30周龄猪的关节软骨和骺软骨进行研究，以观察与年龄相关的变化及骨软骨病。随着猪龄增长，骺软骨和关节软骨的厚度、细胞密度及血管分布均减少。骨软骨病变化包括软骨“栓子”形成，提示局部骨化失败，以及骺软骨分离和间隙形成。观察到与骺分离相关的骺软骨嗜酸性条纹和间隙形成。电子显微镜显示，软骨表面有一层连续的纤维层，对应于光学显微镜下的亮层。随着年龄增长，该层厚度增加，纤维间出现无定形物质积聚。在基质中，胶原纤维的取向和分布随生长而变化，较粗且有清晰亚带的纤维在老龄组中更为常见。此外，在老年动物正常软骨基质中还发现坏死细胞、含糖原小体和细胞碎片。年轻软骨中的软骨细胞显示负责蛋白质合成的细胞器积聚；而在老龄软骨中则观察到高尔基体、囊泡、溶酶体、发育良好的足突和其他内含物。软骨侵蚀在表面有聚集和破坏的亮层，基质和软骨细胞胞质的基质中有电子透明斑块。
