Kupchik H Z, Zurawski V R, Hurrell J G, Zamcheck N, Black P H
Cancer Res. 1981 Sep;41(9 Pt 1):3306-10.
Hybridoma cell lines secreting monoclonal antibodies to carcinoembryonic antigen (CEA) were generated by fusing mouse immune lymphocytes with the mouse myeloma variant cell line, NS-1. Antibody secreted by one cloned hybrid cell line could bind only a select portion of the CEA bound by the commercially available goat anti-CEA antiserum used in clinical assays. Radiolabeled CEA could be purified on a monoclonal antibody affinity column. Incorporation of this purified radiolabeled CEA in a double-antibody solid-phase assay with goat anti-CEA antiserum led to an approximately 2.5-fold increase in sensitivity of the assay. Genetically stable hybrid clones may be sources of virtually unlimited quantities of such antibodies which may have potential utility in improving the cancer specificity of clinical assays.
通过将小鼠免疫淋巴细胞与小鼠骨髓瘤变异细胞系NS-1融合，产生了分泌针对癌胚抗原（CEA）的单克隆抗体的杂交瘤细胞系。一个克隆的杂交细胞系分泌的抗体只能结合临床检测中使用的市售山羊抗CEA抗血清所结合的CEA的特定部分。放射性标记的CEA可在单克隆抗体亲和柱上纯化。将这种纯化的放射性标记的CEA加入到与山羊抗CEA抗血清的双抗体固相检测中，导致检测灵敏度提高了约2.5倍。基因稳定的杂交克隆可能是几乎无限量此类抗体的来源，这些抗体可能在提高临床检测的癌症特异性方面具有潜在用途。
