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从大肠杆菌中产生的免疫球蛋白多肽链生成抗体活性。

Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.

作者信息

Cabilly S, Riggs A D, Pande H, Shively J E, Holmes W E, Rey M, Perry L J, Wetzel R, Heyneker H L

出版信息

Proc Natl Acad Sci U S A. 1984 Jun;81(11):3273-7. doi: 10.1073/pnas.81.11.3273.

DOI:10.1073/pnas.81.11.3273
PMID:6374653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345489/
Abstract

Plasmids have been constructed that direct the synthesis in Escherichia coli of heavy chains and/or light chains of an anti-carcinoembryonic antigen (CEA) antibody. Another plasmid was constructed for expression of a truncated form of heavy chain (Fd' fragment) in E. coli. Functional CEA-binding activity was obtained by in vitro reconstitution in E. coli extracts of heavy chain or Fd' fragment mixed with extracts containing light chain.

摘要

已经构建了质粒，可指导在大肠杆菌中合成抗癌胚抗原（CEA）抗体的重链和/或轻链。还构建了另一种质粒，用于在大肠杆菌中表达截短形式的重链（Fd'片段）。通过在大肠杆菌提取物中体外重组重链或Fd'片段与含有轻链的提取物混合，获得了具有功能的CEA结合活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92f/345489/1ecd07a1ad67/pnas00612-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92f/345489/1ecd07a1ad67/pnas00612-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92f/345489/1ecd07a1ad67/pnas00612-0022-a.jpg

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