Mohla S, Ampy F R, Sanders K J, Criss W E
Cancer Res. 1981 Sep;41(9 Pt 2):3821-3.
The role of androgens in the regulation of carcinogen metabolism in the renal tissue of BALB/c mice was investigated. Kidney microsomal enzyme preparations from mature and immature animals were used in mutagenic studies using the Ames test. Androgen receptors (cytosolic and nuclear) were also evaluated. The results show that the microsomal enzymes from mature males had greater potential to biotransform dimethylnitrosamine than did the microsomal enzymes from mature females or immature animals. Testosterone treatment of mature females or immature animals resulted in a significant increase in the mutagenic ability of their renal microsomal enzymes. Androgen receptors were detected in kidney cytosols of mature and immature animals (both males and females); however, nuclear androgen receptors were detected only in the mature males. Testosterone treatment resulted in a significant accumulation of nuclear androgen receptors in the kidneys of mature females and immature animals. The relationships among mutagenic activity, androgen receptors, the levels of N-demethylase (an enzyme responsible for conversion of dimethylnitrosamine to its active metabolite), dietary fat, and the carcinogen metabolism are discussed.
研究了雄激素在调节BALB/c小鼠肾组织中致癌物代谢的作用。使用Ames试验，将来自成熟和未成熟动物的肾脏微粒体酶制剂用于致突变性研究。还评估了雄激素受体（胞质和核内）。结果表明，成熟雄性动物的微粒体酶比成熟雌性动物或未成熟动物的微粒体酶具有更大的生物转化二甲基亚硝胺的潜力。对成熟雌性动物或未成熟动物进行睾酮处理后，其肾脏微粒体酶的致突变能力显著增加。在成熟和未成熟动物（雄性和雌性）的肾脏胞质溶胶中均检测到雄激素受体；然而，仅在成熟雄性动物中检测到核雄激素受体。睾酮处理导致成熟雌性动物和未成熟动物肾脏中核雄激素受体显著积累。讨论了致突变活性、雄激素受体、N-脱甲基酶（一种负责将二甲基亚硝胺转化为其活性代谢物的酶）水平、膳食脂肪和致癌物代谢之间的关系。
