Ampy F R, Williams A O
Life Sci. 1986 Sep 8;39(10):923-30. doi: 10.1016/0024-3205(86)90374-7.
The potential of hepatic and renal homogenates from three inbred strains of mice (BALB/c, C57BL and DBA) to activate dimethylnitrosamine (DMN) was investigated. Microsomal enzyme (S-9) preparations of liver and kidney from mature and immature mice were used in the Ames Salmonella mutagenicity assay. No age or sex-related differences in the formation of active mutagenic DMN Metabolites by liver microsomal enzymes were observed within any of the three inbred strains. In contrast, mature male kidney S-9 fractions from all three strains had a significantly greater potential to activate DMN than mature female and immature animals. Testosterone treatment resulted in no apparent changes in the ability of hepatic tissue to biotransform DMN to its mutagenic metabolites among age and sex classes. However, after testosterone treatment, renal microsomal fractions from mature female mice of all three strains did not differ significantly from their male counterparts in their ability to transform DMN to mutagenic metabolites.
研究了三种近交系小鼠（BALB/c、C57BL和DBA）的肝匀浆和肾匀浆激活二甲基亚硝胺（DMN）的潜力。在Ames沙门氏菌诱变性试验中，使用了来自成熟和未成熟小鼠肝脏和肾脏的微粒体酶（S-9）制剂。在这三个近交系中的任何一个系内，均未观察到肝微粒体酶形成活性诱变DMN代谢产物时存在年龄或性别相关差异。相比之下，来自所有三个品系的成熟雄性肾脏S-9组分激活DMN的潜力明显大于成熟雌性和未成熟动物。睾酮处理并未导致肝组织在不同年龄和性别类别中将DMN生物转化为诱变代谢产物的能力出现明显变化。然而，睾酮处理后，所有三个品系成熟雌性小鼠的肾微粒体组分在将DMN转化为诱变代谢产物的能力上与雄性对应物没有显著差异。
