Lutsenko N I, Mel'nichuk D A, Zhuravskiĭ N I
Ukr Biokhim Zh (1978). 1978 Sep-Oct;50(5):635-40.
A rise in the carbonic acid and pH level in rat blood determines an increase in oxidative properties of the liver cell cytoplasm. Simultaneously the content of alpha-ketoglutarate, isocitrate, glutamate, alpha-glycerophosphate, dihydroxyacetone phosphate, pyruvate, lactate, malate increases and the level of glucose, glutamine and ammonia decreases. The in vitro experiments showed that the rise in the level of carbonic acid in the incubation medium from 10 up to 40 mM under all the studied values of pH (7.2, 7.4, 7.6) stimulates formation of dihydroxyacetone phosphate, malate, pyruvate, alpha-ketoglutarate (by 1.5-2.0 times). The same regularity is observed for lactate and glutamate at pH 7.4 and 7.6. The increase in the level of carbonic acid under the all studied values of pH produces a 1.5-fold decrease in the ammonia concentration.
大鼠血液中碳酸和pH值的升高会导致肝细胞细胞质氧化特性增强。同时，α-酮戊二酸、异柠檬酸、谷氨酸、α-磷酸甘油、磷酸二羟丙酮、丙酮酸、乳酸、苹果酸的含量增加，而葡萄糖、谷氨酰胺和氨的水平降低。体外实验表明，在所有研究的pH值（7.2、7.4、7.6）下，孵育培养基中碳酸水平从10 mM升高到40 mM会刺激磷酸二羟丙酮、苹果酸、丙酮酸、α-酮戊二酸的生成（增加1.5 - 2.0倍）。在pH 7.4和7.6时，乳酸和谷氨酸也呈现相同规律。在所有研究的pH值下，碳酸水平的升高会使氨浓度降低1.5倍。
