[Effect of carbonic acid of different concentrations of the glycolysis processes and tricarboxylic acid cycle in rat liver tissues].

A rise in the carbonic acid and pH level in rat blood determines an increase in oxidative properties of the liver cell cytoplasm. Simultaneously the content of alpha-ketoglutarate, isocitrate, glutamate, alpha-glycerophosphate, dihydroxyacetone phosphate, pyruvate, lactate, malate increases and the level of glucose, glutamine and ammonia decreases. The in vitro experiments showed that the rise in the level of carbonic acid in the incubation medium from 10 up to 40 mM under all the studied values of pH (7.2, 7.4, 7.6) stimulates formation of dihydroxyacetone phosphate, malate, pyruvate, alpha-ketoglutarate (by 1.5-2.0 times). The same regularity is observed for lactate and glutamate at pH 7.4 and 7.6. The increase in the level of carbonic acid under the all studied values of pH produces a 1.5-fold decrease in the ammonia concentration.