Böhm N, Kröner B, Kaiser E
Cell Tissue Kinet. 1981 Jul;14(4):433-44. doi: 10.1111/j.1365-2184.1981.tb00550.x.
After Feulgen staining with acriflavine-Schiff, the DNa content of glial and neuronal nuclei from various sites of the human CNS (pre- and post-central gyrus, cerebellar cortex and spinal cord) were determined by fluorescence cytophotometry. The specimens were obtained from twelve adult human autopsy cases. Glial cell nuclei always revealed a biomodal DNA distribution pattern with a large 2c and a smaller 4c peak. The 4c peak was most prominent in the cerebellum. A few 8c glial nuclei were found. Neuronal cell nuclei disclosed DNA histograms with hyperdiploid means in the range 2.2-2.5c (1.8-2.9c for the individual populations). Tetraploid 4c DNA values were not observed, neither in Purkinje cells, nor in pyramidal cells. In eleven out of a total of forty-four slides the higher DNA means of neuronal nuclei were found to be statistically significant (P greater than 0.05) when compared with a population of 2c hepatocytes on the same slide. The results indicate the existence of some 'extra DNA' in human neuronal cell nuclei, the biological significance of which has still to be elucidated. It is however, suggested that it may play an important role in the functional activity of the CNS.
在用吖啶黄 - 席夫试剂进行福尔根染色后，通过荧光细胞光度法测定了来自人类中枢神经系统不同部位（中央前回和中央后回、小脑皮质和脊髓）的神经胶质细胞核和神经元核的DNA含量。标本取自12例成人尸检病例。神经胶质细胞核总是呈现出一种双峰DNA分布模式，有一个较大的2c峰和一个较小的4c峰。4c峰在小脑中最为明显。发现了一些8c神经胶质细胞核。神经元核的DNA直方图显示超二倍体平均值在2.2 - 2.5c范围内（各个群体为1.8 - 2.9c）。在浦肯野细胞和锥体细胞中均未观察到四倍体4c DNA值。在总共44张载玻片中，有11张载玻片上神经元核的较高DNA平均值与同一张载玻片上的2c肝细胞群体相比具有统计学意义（P大于0.05）。结果表明人类神经元细胞核中存在一些“额外的DNA”，其生物学意义尚待阐明。然而，有人认为它可能在中枢神经系统的功能活动中起重要作用。
